2023年济南市7例猴痘病毒全基因组特征分析  

Whole-genome characterization of MPXV strains isolated from local cases in Ji′nan City in 2023

作　　者：赵红[1] 刘子薇 张钦钰 刘娜 刘岚铮[1] 焦海涛[1] ZHAO Hong;LIU Ziwei;ZHANG Qinyu;LIU Na;LIU Lanzheng;JIAO Haitao(Ji′nan Center for Disease Control and Prevention,Ji′nan 250021,China;Gangcheng District Center for Disease Control and Prevention,Ji′nan 271100,China)

机构地区：[1]济南市疾病预防控制中心,济南250021 [2]济南市钢城区疾病预防控制中心,济南271100

出　　处：《中国公共卫生》2024年第5期632-634,共3页Chinese Journal of Public Health

摘　　要：目的分析山东省济南市猴痘病毒(MPXV)基因型别及基因组特征。方法对2023年6—10月济南市上报疑似感染猴痘病毒病例的血清和痘疱液样本进行猴痘病毒核酸检测。利用全基因组测序技术获得猴痘病毒全基因组序列。应用Mega 11软件、Bioedit软件、Nextclade在线比对网站对猴痘病毒基因组序列进行分子遗传进化分析,建立系统进化树和核苷酸及氨基酸序列突变分析。结果本研究通过全基因测序技术获得7例济南市猴痘病毒全基因组序列,基因组全长196858 bp,上传Nextclade网站在线比对结果均为西非分支IIb分支,谱系分型为C.1。核苷酸变异深度>50%个数为86个,变异影响≥中级总个数为38个。结论济南市猴痘病毒与中国境内猴痘病毒毒株序列进化距离较近属于同一进化分支,无新分支发现。Objective To analyze the genomic characteristics of the monkeypox virus(MPXV)strains isolated from local cases in Ji′nan city,Shandong province.Methods Blood serum and vesicular fluid samples from suspected cases of MPXV infection reported in Ji′nan city from June to October 2023 were tested for MPXV nucleic acids.The complete genome sequence of MPXV was obtained using whole-genome sequencing(WGS)technology.Molecular genetic evolution of the MPXV genome sequence was performed using Mega 11 software,Bioedit software,and the Nextclade online comparison website.Systematic phylogenetic trees and nucleotide and amino acid sequence mutation analyses were performed.Results In this study,the complete genome sequences of 7 strains of MPXV isolated from the cases in Ji′nan city were obtained by WGS,with a total genome length of 196858 bp.The online comparison results uploaded to the Nexttrade website showed that all of them belonged to the IIb branch of the West African branch,and the lineage classification was C.1.The number of nucleotide variations with a depth greater than 50%is 86,and the total number of variations with an effect of≥intermediate is 39.Conclusion The evolutionary distance between the sequences of MPXV strains isolated in Ji′nan city and those in China is relatively close,belonging to the same evolutionary branch,and no new branches have been found.

关 键 词：猴痘 猴痘病毒 基因型别 进化分析 济南 

分 类 号：R373[医药卫生—病原生物学]