赤芍通过PGC-1α/Nrf2对人脐静脉内皮细胞促血管新生作用研究  

Paeoniae Promotes Angiogenesis in OGD-mediated HUVECs Through the PGC-1α/Nrf2 Pathway

作　　者：陈敏纯 薛润青 郑洁[1] 赵新 李颖[1] 张宏丽 CHEN Minchun;XUE Runqing;ZHENG Jie;ZHAO Xin;LI Ying;ZHANG Hongli(Department of Pharmacy,Xi'an No.3 Hospital,the Affiliated Hospital of Northwest University,Xi'an 710018,China;Department of Pharmacy,the Hospital of Xidian University,Xi'an 710000,China;Department of Pharmacy,Xi'an Central Hospital,Xi'an 710001,China)

机构地区：[1]西北大学附属医院/西安市第三医院药剂科,陕西西安710018 [2]西安电子科技大学医院药剂科,陕西西安710000 [3]西安市中心医院药剂科,陕西西安710001

出　　处：《现代中药研究与实践》2024年第3期22-26,共5页Research and Practice on Chinese Medicines

基　　金：陕西省自然科学基础研究计划项目(2023-JC-YB-657);西安市科技计划项目(21YXYJ0040);西安市中医药项目(SZJ202104);西安市卫生健康委员会项目(2023qn05)。

摘　　要：目的 探讨赤芍对人脐静脉内皮细胞氧糖剥夺再灌注损伤后促血管新生的作用机制。方法 将人脐静脉内皮细胞随机分为空白对照组、模型组、赤芍含药血清组,采用氧糖剥夺再灌注损伤模型,分析人脐静脉内皮细胞缺血缺氧损伤后,赤芍对细胞增殖、迁移、成管能力、Nrf2、PGC-1α蛋白表达以及凋亡情况。结果 与模型组比较,赤芍含药血清组促进人脐静脉内皮细胞增殖、提高细胞粘附率、迁移率、成管能力、促进Nrf2、PGC-1α蛋白表达、抑制细胞凋亡(P<0.05)。结论 赤芍通过PGC-1α/Nrf2信号通路促进人脐静脉内皮细胞缺血缺氧损伤后的血管新生作用。Objective To evaluate the mechanism of paeoniae on angiogenesis in oxygen-glucose deprivation (OGD)-induced injury of human umbilical vein endothelial cells (HUVECs).Methods HUVECs were randomly divided into sham group,model group,and the paeoniae containing serum group.The capacity of cell proliferation,migration,tube formation,apoptosis of HUVECs,and PGC-1α/Nrf2 protein expression methods was evaluated after paeoniae treatment,respectively.Results Paeoniae administration enhanced the capacity of cell proliferation,migration,tube formation,reduced apoptosis of HUVECs,and increased expression level of PGC-1α/Nrf2 protein(P < 0.05).Conclusion Paeoniae can reduce OGD-mediated HUVECs injury by promoting angiogenesis through the PGC-1α/Nrf2 pathway.

关 键 词：赤芍 血管新生 氧糖剥夺模型 PGC-1α/Nrf2通路 人脐静脉内皮细胞 

分 类 号：R285.5[医药卫生—中药学]