万丈深提取物止咳平喘及抗炎作用研究  

Anti-tussive,Anti-asthmatic and Anti-inflammatory Effects of Crepis phoenix Dunn Extract

作　　者：詹易 何志明 黄加文 吴德松 李小辉 赵建军 查雨锋 ZHAN Yi;HE Zhiming;HUANG Jiawen;WU Desong;LI Xiaohui;ZHAO Jianjun;ZHA Yufeng(Yunnan Institute of Materia Medica,Kunming 650111,China;Yunnan Province Company Key Laboratory for TCM and Ethnic Drug of New Drug Creation,Kunming 650111,China)

机构地区：[1]云南省药物研究所,云南昆明650111 [2]云南省中药和民族药新药创制企业重点实验室,云南昆明650111

出　　处：《现代中药研究与实践》2024年第3期34-39,共6页Research and Practice on Chinese Medicines

基　　金：云南特色植物筛选与研发服务CXO平台建设(2022YKZY001)。

摘　　要：目的 研究万丈深的止咳平喘及抗炎作用。方法 采用小鼠急性毒性实验评价万丈深提取物的安全性;氨水引咳小鼠模型、枸橼酸引咳豚鼠模型及乙酰甲胆碱联合磷酸组胺引喘豚鼠模型研究万丈深的止咳平喘作用;脂多糖(LPS)诱导小鼠单核巨噬细胞(RAW264.7)炎症模型研究万丈深的体外抗炎活性。结果 万丈深水提物、醇提物小鼠灌胃给药急性毒性试验最大给药量分别为40.0 g/kg、60.0 g/kg,万丈深提取物能明显减少小鼠及豚鼠咳嗽次数,延长引咳潜伏期及豚鼠引喘潜伏期,降低RAW264.7炎症细胞中IL-6及TNF-α含量。结论 万丈深醇提物的止咳平喘效果较水提物好,其止咳平喘作用可能与抑制有关炎症反应的发生相关。Objective To investigate the anti-tussive,anti-asthmatic and anti-inflammatory effects of Crepis phoenix Dunn.Methods Acute toxicity test was used to study the safety of Crepis phoenix Dunn extract in mice.Ammonia water induced cough mouse model,citric acid induced cough guinea pig model and methacholine combined with histamine phosphate induced asthma guinea pig model were used to evaluate the antiasthmatic effect of Crepis phoenix Dunn.To study the anti-inflammatory activity of Crepis phoenix Dunn in vitro by lipopolysaccharide (LPS)-induced mouse monocyte-macrophage (RAW264.7) inflammatory model.Results The maximum doses of Crepis phoenix Dunn water extract and alcohol extract were 40.0 g/kg and 60.0 g/kg,respectively,in the acute toxicity test of mouse by gavage.Crepis phoenix Dunn extract could significantly reduce the number of coughs in mouse and guinea pigs,prolong the latency of cough induction in mouse and asthma induction in guinea pigs,and reduce the content of IL-6 and TNF-α in RAW264.7 inflammatory cells.Conclusion The anti-tussive,anti-asthmatic and anti-inflammatory effects of Crepis phoenix Dunn alcohol extract are better than water extract,and its anti-tussive and anti-asthmatic effect may be related to inhibiting the occurrence of related inflammatory reactions.

关 键 词：万丈深 止咳 平喘 抗炎 豚鼠 小鼠 

分 类 号：R285.5[医药卫生—中药学]