姜黄素减肥胶囊中功效成分含量测定方法学验证实验设计  

Method Validation Experimental Design for the Determination of Functional Ingredient in Curcumin Jianfei Capsules

作　　者：郑海云[1] 王少南 赵海誉[2] 李光敏 吕燕[1] 王斌[1] ZHENG Hai-Yun;WANG Shao-Nan;ZHAO Hai-Yu;LI Guang-Min;LV Yan;WANG Bin(Cooperation Center of Traditional Chinese Medicine Science and Technology,China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Traditional Chinese Medicine,China Academy of Traditional Chinese Medicine,Beijing 100700,China)

机构地区：[1]中国中医科学院中医药科技合作中心,北京100700 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国药物经济学》2024年第7期89-92,96,共5页China Journal of Pharmaceutical Economics

基　　金：中国中医科学院科技创新工程项目(CI2021A04510)。

摘　　要：目的设计姜黄素减肥胶囊中功效成分姜黄素含量测定方法学验证综合试验。方法以十八烷基硅烷键合硅胶为填充剂,乙腈-4%冰醋酸(48∶52)为流动相,检测波长430 nm,流速为1 ml/min进行检测,以峰面积外标法定量。结果在选定的色谱条件下,本方法的专属性、线性、精密度、准确度、稳定性满足要求。结论经过方法学验证,本方法简便快捷、准确度高、重复性好,是检测姜黄素减肥胶囊中姜黄素的有效定量方法。Objective To design method validation for the determination of the content of curcumin in curcumin jianfei capsules.Methods Using Acetonitrile and 4% Acetic acid(48∶52) as mobile phase,separated by Carotenoid column,detected by HPLC at 430 nm,and quantified by the external standard method.Results Method validation included exclusivity,linearity,precision,accuracy and stability,which laid the foundation for the control the quality and assay the content of functional ingredient in curcumin Jianfei capsules.Conclusions he method is simple,rapid and accurate,which can be applied to the analysis of the content of curcumin in curcumin Jianfei capsulles.

关 键 词：含量测定 姜黄素 方法学验证 实验设计 

分 类 号：R286.0[医药卫生—中药学]