抗真菌药物敏感性检测新方法的研究进展  

Research progress on new methods for antifungal drug susceptibility testing

作　　者：陈燕珊(综述) 芮勇宇(审校)[1] CHEN Yanshan;RUI Yongyu(Department of Medical Laboratory,Nanfang Hospital,Southern Medical University,Guangzhou,Guangdong 510515,China)

机构地区：[1]南方医科大学南方医院检验医学科,广东广州510515

出　　处：《检验医学与临床》2024年第17期2602-2606,共5页Laboratory Medicine and Clinic

基　　金：广东省自然科学基金项目(2021A1515012249)。

摘　　要：抗真菌药物敏感性检测的目标是生成可靠的最小抑菌药物浓度,判断菌株的药物敏感性,从而指导临床医生的抗真菌治疗,为流行病学研究和开发新的抗真菌药物提供信息。然而目前的标准化药物敏感性检测方法与许多商品化药物敏感性检测方法存在检测时间长、适用菌株范围不完整的局限性。该文对目前的抗真菌药敏试验新方法进行综述,包括单细胞水平分析法、基质辅助激光解吸电离飞行时间质谱、等温微量热法及新型的商品化检测系统,并对它们的优点和不足进行分析,以期在日益加重的全球真菌感染中为抗真菌药物敏感性检测新方法提供新的研究方向,实现快速、准确的药物敏感性检测。The goal of antifungal sensitivity detection is to generate a reliable minimum concentration of antibacterial drugs,to judge the drug sensitivity of strains,to guide clinicians in antifungal treatment,to provide information for epidemiological research and to develop new antifungal drugs.However,the current standardized drug sensitivity testing methods and many commercial drug sensitivity detection methods have the limitations of long detection time and incomplete range of applicable strains.In this paper,the new methods of antifungal susceptibility test are reviewed,including single cell level analysis,matrix-assisted laser desorption ionization time-of-flight mass spectrometry and isothermal microcalorimetry detection and new commercial detection systems.Their advantages and disadvantages are analyzed to provide a new research direction for new methods of antifungal susceptibility detection in the increasing global fungal infections and to achieve rapid and accurate drug sensitivity detection.

关 键 词：药物敏感性检测 抗真菌药物 耐药 快速检测 最小抑菌药物浓度 

分 类 号：R446.5[医药卫生—诊断学]