新型驱避配方的筛选及对白纹伊蚊室内驱避效果研究  

Screening for a new mosquito repellent formula and its repellent effect against Aedes albopictus in laboratory

作　　者：刘曜[1] 刘锐 张杰[2] 姚隽一 张宸罡 李鑫 吴寰宇[1] 刘洪霞[1] LIU Yao;LIU Rui;ZHANG Jie;YAO Jun-yi;ZHANG Chen-gang;LI Xin;WU Huan-yu;LIU Hong-xia(Department of Vector Control and Prevention,Division of Infectious Diseases Control and Prevention,Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China;Shanghai Yangpu District Center for Disease Control and Prevention,Shanghai 200090,China;BASF(China)Co.,Ltd,Shanghai 200003,China)

机构地区：[1]上海市疾病预防控制中心传染病防治所病媒生物防治科,上海200336 [2]上海市杨浦区疾病预防控制中心,上海200090 [3]巴斯夫(中国)有限公司,上海200003

出　　处：《中国媒介生物学及控制杂志》2024年第4期411-416,共6页Chinese Journal of Vector Biology and Control

基　　金：上海市加强公共卫生体系建设三年行动计划(2023-2025年)重点学科(GWⅥ-11.1-13)。

摘　　要：目的探索新型驱避成分不同浓度的实验室驱避药效,比较加入助剂的配方制剂与其他常用驱避剂成分对白纹伊蚊的室内驱避效果,为新型驱避剂的研发提供实验基础。方法采用《农药登记用卫生杀虫剂室内药效试验及评价第9部分:驱避剂》(GB/T 13917.9—2009)试验方法。利用Excel 2007软件进行数据录入,使用SPSS 22.0软件进行统计学分析。采用重复测量多因素方差分析检验不同浓度新型驱避剂和乙醇对蚊虫的驱避时间是否存在差异;采用配对样本t检验对比乙醇配制的制剂与新型驱避剂的配方制剂间驱避时间差异;采用随机区组设计方差分析检验配方制剂与常用驱避剂对蚊虫的驱避时间差异。结果新型驱避成分对白纹伊蚊的驱避效果随浓度的升高而增强。新型驱避剂的浓度达到10%时驱避时间可达到4 h以上,浓度为20%时驱避时间可以达到6 h以上。5%浓度制剂[平均驱避时间为(2.62±0.83)h]的驱避效果低于12%、15%和20%等3组驱避剂浓度[平均驱避时间分别为(5.13±1.05)、(4.94±0.98)和(6.08±1.76)h,均P<0.05],其余各浓度驱避剂的驱避时间差异无统计学意义[10%浓度制剂的平均驱避时间为(4.91±1.17)h,均P>0.05]。15%乙醇制剂[平均驱避时间为(4.16±1.56)h]的驱避效果低于50%乙醇制剂[平均驱避时间为(5.22±1.53)h]的驱避效果(P<0.05),其余各浓度乙醇制剂的驱避时间差异无统计学意义[35%和75%乙醇制剂的平均驱避时间分别为(4.69±1.59)和(4.89±1.77)h,均P>0.05]。新型驱避成分与乙醇的交互效应不显著(F=0.849,P=0.601)。使用助剂能延长驱避时间,新型驱避剂配方制剂的驱避时间比12%终浓度的15%、35%、50%和75%乙醇制剂分别增长了55.25%、16.73%、11.89%和9.91%。配方制剂[驱避时间为(6.21±0.97)h]与常用驱避剂对比,驱避效果优于4.5%驱蚊酯[驱避时间为(2.89±0.83)h]和7%避蚊胺[驱避时间为(3.54±0.96)h],低于10%羟哌酯[驱避时�Objective To explore the repellent effects of a new mosquito repellent at different concentrations in laboratory,and to compare the indoor repellent effects of formula with additives and other commonly used repellent components on Aedes albopictus,so as to provide an experimental basis for the development of new repellents.Methods The experimental methods were based on the Laboratory Efficacy Test Methods and Criterions of Public Health Insecticides for Pesticide Registration-Part 9:Repellent(GB/T 13917.9-2009).Excel 2007 software was used for data entry and SPSS 22.0 software for statistical analysis.Multivariate repeated measures analysis of variance was used to test the difference in the repellent time of the new repellent and ethanol at various concentrations.The paired samples t-test was used to compare the difference in the repellent time between ethanol solution and the new repellent formula.Random block design and analysis of variance were used to test the differences in the repellent time of the new repellent formula and commonly used repellents against mosquitoes.Results The repellent effect of the new repellent increased with increasing concentration.When the concentration of new repellent was 10%,the repellent time was more than 4 h,and when the concentration was 20%,the repellent time was more than 6 h.The repellent effect of 5%concentration[average repellent time(2.62±0.83)h]was lower than those of 12%,15%,and 20%concentrations[average repellent time(5.13±1.05),(4.94±0.98),and(6.08±1.76)h,all P<0.05].There was no significant difference in the repellent time of new repellent at other concentrations[average repellent time(4.91±1.17)h for 10%concentration,all P>0.05].The repellent effect of formula with 15%ethanol[average repellent time(4.16±1.56)h]was lower than that of the formula with 50%ethanol[average repellent time(5.22±1.53)h,P<0.05].There was no significant difference in the repellent time of formulas with other ethanol concentrations[average repellent time(4.69±1.59)h for 35%ethanol an

关 键 词：驱避剂 白纹伊蚊 避蚊胺 羟哌酯 驱蚊酯 孟二醇 

分 类 号：R384.1[医药卫生—医学寄生虫学] S481.9[医药卫生—基础医学]