鼠妇内生真菌Talaromyces malicola的次生代谢产物研究  

Secondary metabolites of endophytic fungus Talaromyces malicola hosted in Armadillidium vulgare

作　　者：杨晨宇 陈科良 刘洋 易诚 贾兴宝 刘云宝 YANG Chen-yu;CHEN Ke-liang;LIU Yang;YI Cheng;JIA Xing-bao;LIU Yun-bao(State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100050,China)

机构地区：[1]中国医学科学院北京协和医学院药物研究所天然药物活性物质与功能国家重点实验室,北京100050

出　　处：《中国中药杂志》2024年第16期4470-4476,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(22207127)。

摘　　要：对节肢动物鼠妇内生真菌Talaromyces malicola的次生代谢产物进行研究,采用硅胶柱色谱、凝胶柱色谱、半制备液相色谱等进行分离纯化,共从鼠妇内生真菌T.malicola发酵物的乙酸乙酯部位中分离鉴定出11个化合物。结合核磁共振波谱、高分辨质谱、紫外、红外以及圆二色谱等分析方法确定了化合物的结构,分别为talarosesquiterpene A(1)、(3β,5α,6α,15α,22E)-5,6-epoxyergosta-8(14),22-diene-3,7,15-triol(2)、vermistatin(3)、hydroxyvermistatin(4)、bercheminol A(5)、penicillide(6)、lunatinin(7)、penipurdin A(8)、emodin(9)、BE-25327(10)、(-)-regiolone(11)。化合物1为新的diaporol型倍半萜类化合物,化合物2、4~5、7~11为首次从篮状菌属真菌中分离得到。The secondary metabolites of the endophytic fungus Talaromyces malicola hosted in the arthropod Armadillidium vulgare were separated by silica gel column chromatography,gel column chromatography,and semi-preparative high-performance liquid chromatography.Eleven compounds(1-11)were obtained from the ethyl acetate fraction of the fermentation broth of T.malicola,and their structures were identified by NMR,HR-ESI-MS,UV,IR,and ECD.The 11 compounds were talarosesquiterpene A(1),(3β,5α,6α,15α,22E)-5,6-epoxyergosta-8(14),22-diene-3,7,15-triol(2),vermistatin(3),hydroxyvermistatin(4),bercheminol A(5),penicillide(6),lunatinin(7),penipurdin A(8),emodin(9),BE-25327(10),and(-)-regiolone(11).Compound 1 was a new diaporol-type sesquiterpene.Compounds 2,4-5,and 7-11 were isolated from Talaromyces for the first time.

关 键 词：鼠妇 内生真菌 Talaromyces malicola 次生代谢产物 

分 类 号：R284[医药卫生—中药学]