酪氨酸酶、过氧化物酶和碱性磷酸酶基因在皱纹盘鲍初生壳形成中的表达分析  

Expression patterns of tyrosinase,peroxidase and alkaline phosphatase genes during larval shell formation of the mollusk Haliotis discus hannai

作　　者：殷晓雯 谭素建 许飞[1,2] 郇聘 刘保忠[1,2] YIN Xiaowen;TAN Sujian;XU Fei;HUAN Pin;LIU Baozhong(Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院海洋研究所,实验海洋生物学重点实验室,山东青岛266071 [2]中国科学院大学,北京100049

出　　处：《水产学报》2024年第9期76-84,共9页Journal of Fisheries of China

基　　金：国家自然科学基金(32273128);现代农业产业技术体系建设专项(CARS-49)。

摘　　要：为研究软体动物贝壳发育区形成机制,实验通过扫描电子显微镜观察分析了皱纹盘鲍贝壳发育区的形态特征,鉴定了皱纹盘鲍的酪氨酸酶(Hdi-Tyr1和Hdi-Tyr2)、过氧化物酶(Hdi-Prx1、Hdi-Prx2)和碱性磷酸酶(Hdi-Alp)等5个贝壳形成相关的候选基因,并通过整装原位杂交技术解析了各基因在皱纹盘鲍早期幼虫发育过程中的表达模式。结果显示,皱纹盘鲍贝壳发育区由至少3种特征差异明显的细胞组成,酪氨酸酶基因(Hdi-Tyr1和HdiTyr2)在担轮幼虫的贝壳发育区呈“U”形表达,在面盘幼虫外套膜的长柱状细胞中表达,而碱性磷酸酶(Hdi-Alp)和过氧化物酶(Hdi-Prx1和Hdi-Prx2)基因仅表达在幼虫的担轮环和头部。研究表明,Hdi-Tyr1和Hdi-Tyr2基因可能参与了皱纹盘鲍担轮幼虫和面盘幼虫的贝壳形成过程,而过氧化物酶基因Hdi-Prx1和Hdi-Prx2以及碱性磷酸酶基因Hdi-Alp并不参与初生壳的发育过程,实验结果为深入解析软体动物的贝壳发育机制和贝壳相关性状的种质创新提供了基础支撑。To elucidate the mechanisms underlying larval shell development,this study examined the morpholo-gical traits of shell field in the Pacific abalone Haliotis discus hannai using scanning electron microscopy and assessed the expression profiles of five candidate genes related to shell formation,including tyrosinases gene(Hdi-Tyr1,Hdi-Tyr2),peroxidases gene(Hdi-Prx1,Hdi-Prx2),and alkaline phosphatase gene(Hdi-Alp).The results revealed that the shell field consisted of at least three distinct cell populations displaying a characteristic"rosette"pattern.Expression of the Hdi-Tyr1 and Hdi-Tyr2 displayed U-shaped patterns in the shell field of trochophore lar-vae,and their mRNA were identified in columnar cells at the mantle edge of veliger larvae.However,Hdi-Alp,Hdi-Prx1 and Hdi-Prx2 were exclusively expressed in the prototroch and head of the larvae and showed no detectable expression in the shell field.These findings indicate that Hdi-Tyr1 and Hdi-Tyr2 may be involved in the larval shell formation of H.discus hannai,whereas Hdi-Prx1,Hdi-Prx2 and Hdi-Alp are not involved in this process.These insights provide a foundation for subsequent research into the shell development mechanisms and genetic improvement of shell-related traits in mollusks.

关 键 词：皱纹盘鲍 贝壳发育区 酪氨酸酶 过氧化物酶 碱性磷酸酶 

分 类 号：Q786[生物学—分子生物学] S968.3[农业科学—水产养殖]