外源物质对盐碱胁迫下垂丝海棠生长及生理特性的影响  

Effects of Exogenous Substances on the Growth and Physiological Characteristics of Malus halliana under Salt-alkali Stress

作　　者：达建东 郭润霞 高立杨 李平 DA Jiandong;GUO Runxia;GAO Liyang;LI Ping(Wuwei Agricultural Technology Promotion Centre,Wuwei Gansu 733000,China;Wuwei Academy of Agricultural Sciences,Wuwei Gansu 733000,China;Wuwei Agriculture and Rural Bureau,Wuwei Gansu 733000,China)

机构地区：[1]武威市农业技术推广中心,甘肃武威733000 [2]武威市农业科学研究院,甘肃武威733000 [3]武威市农业农村局,甘肃武威733000

出　　处：《寒旱农业科学》2024年第8期734-739,共6页Journal of Cold-Arid Agricultural Sciences

基　　金：武威市自然科学基金(WW23A03RPZ006)。

摘　　要：为探究褪黑素和2,4-表油菜素内酯在盐碱胁迫下对垂丝海棠生长及其生理特性的影响,以9~10叶龄垂丝海棠实生苗为材料,设置200μmol/L褪黑素喷施、200μmol/L褪黑素+0.2 mg/L 2,4-表油菜素内酯叠加喷施、0.2 mg/L 2,4-表油菜素内酯喷施3个处理,研究其对垂丝海棠盐碱胁迫下叶片叶绿素含量、相对电导率、含水量、抗氧化酶活性、游离脯氨酸含量等生理指标和光合荧光参数的影响。结果表明,外源喷施褪黑素和2,4-表油菜素内酯可显著提高盐碱胁迫下垂丝海棠叶片的相对导电率、脯氨酸含量、抗氧化酶活性,降低叶片相对含水量、叶绿素含量,从而缓解盐碱胁迫对垂丝海棠生长的抑制。本试验中,缓解效果由高到低依次为褪黑素、2,4-表油菜素内酯、二者叠加使用。In order to explore effects of melatonin and 2,4-epibrassinolide to alleviate the damage of combined salt-alkali stress on Malus halliana,in this experiment,the seedlings of Malus halliana with 9 to 10 leaf ages were used as the test material.Three treatments were set,i.e.,200μmol/L melatonin spray,200μmol/L melatonin+0.2 mg/L 2,4-table brassinolide overlay spray and 0.2 mg/L 2,4-table brassinolide spray,to study the effects on chlorophyll content,relative conductivity,water content,antioxidant enzyme activity,free proline content and photosynthetic fluorescence parameters of leaves under salt-alkali stress.The results showed that exogenous application of melatonin and 2,4-epbrassinolide could significantly improve the relative conductivity,proline content and antioxidant enzyme activity of leaves under salt-alkali stress,and reduce the relative water content and chlorophyll content of leaves,so as to alleviate the inhibition of salt-alkali stress on the growth of Malus linus.In this experiment,the alleviating effect from high to low was melatonin,2,4-epbrassinolide,and the combination of the two.

关 键 词：垂丝海棠 盐碱胁迫 褪黑素 2 4-表油菜素内酯 

分 类 号：S661.1[农业科学—果树学]