烟草镰刀菌根腐病生防菌GDND-2的鉴定及防病效果  

The Biocontrol Strain GDND-2 of Tobacco Fusarium Root Rot:Identification and Control Effect

作　　者：沈会芳[1] 杨祁云[1] 张景欣[1] 蒲小明[1] 孙大元[1] 刘平平 林壁润[1] SHEN Huifang;YANG Qiyun;ZHANG Jingxin;PU Xiaoming;SUN Dayuan;LIU Pingping;LIN Birun(Institute of Plant Protection,Guangdong Academy of Agricultural Sciences/Key Laboratory of New Technique for Plant protection in Guangdong,Guangzhou 510640)

机构地区：[1]广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室,广州510640

出　　处：《中国农学通报》2024年第23期126-134,共9页Chinese Agricultural Science Bulletin

基　　金：广东省现代农业产业技术体系创新团队“绿色发展共性关键技术创新团队建设项目”(2022KJ112);广东省烟草科技计划项目“浈江流域上游主要作物病虫害绿色防控研究与应用”(201908)。

摘　　要：为烟草根腐病生防菌剂的研发储备菌株资源。以烟草根腐病菌为靶标,筛选拮抗菌,通过培养形态、生化特征和分子技术鉴定菌株。用平板划线测定拮抗菌耐受力,盆栽试验验证其对病害的防效。筛选出拮抗菌GDND-2,其10%和20%发酵滤液对病菌生长抑制率为53.09%和62.24%。鉴定GDND-2为贝莱斯芽孢杆菌(Bacillus velenzensis),该菌抑菌谱广,耐极端温度、酸碱和盐碱,对多菌灵等7种常用杀菌剂不敏感。GDND-2发酵液处理15、30、45 d,对烟草根腐病的防效为70.00%、74.62%和79.51%,处理45 d的防效高于多菌灵。贝莱斯芽孢杆菌GDND-2抗菌谱广,性状优良,可有效防治烟草根腐病,有望开发为生防菌剂。The study was to reserve resources for the development of new biocontrol agents to control tobacco Fusarium root rot.The antagonistic strains were screened against Fusarium oxysporum,and were identified throgh morphological,biochemical characteristics and molecular technique.The tolerance of the antagonistic strain was determined by plate scribing method,and its control effect on tobacco root rot was verified by potting experiment.The antagonistic strain GDND-2 was screened.Its fermentation filtrate had a strong inhibitory effect on mycelium growth of F.oxysporium,the inhibition rates of 10%and 20%fermentation filtrate were 53.09%and 62.24%.The strain GDND-2 was identified as Bacillus velenzensis.It presented a broad inhibitory spectrum,and showed strong tolerance to extreme temperature,acid-alkali and salt.It was not sensitive to seven commonly used fungicides such as carbendazim.The control effects of fermentation broth of GDND-2 on tobacco root rot were 70.00%,74.62%and 79.51%on the 15th,30th and 45th day after root drenching,and its control effect on the 45th day was higher than carbendazim.Bacillus velenzensis GDND-2 with excellent traits and broad inhibitory spectrum could effectively control tobacco Fusarium root rot,and it is expected to be developed as a biocontrol agent.

关 键 词：烟草镰刀菌根腐病 贝莱斯芽孢杆菌 鉴定 耐受性 防治效果 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]