适配体传感器在抗生素检测中的研究进展  

Research progress of aptasensors for antibiotic detection

作　　者：李晓倩 常洋洋 张强[2] 刘猛 LI Xiaoqian;CHANG Yangyang;ZHANG Qiang;LIU Meng(School of Environmental Science and Technology,Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education),Dalian University of Technology,Dalian,116024,China;School of Bioengineering,Dalian University of Technology,Dalian,116024,China)

机构地区：[1]大连理工大学环境学院,工业生态与环境工程教育部重点实验室,大连116024 [2]大连理工大学生物工程学院,大连116024

出　　处：《环境化学》2024年第8期2598-2615,共18页Environmental Chemistry

基　　金：国家自然科学基金(2019YFC1905401)资助.

摘　　要：抗生素滥用导致的污染以及耐药菌的出现已成为全球性严重问题,对其进行快速、灵敏、即时检测在生态系统和人类健康保护方面具有重要意义.而传统的检测方法,耗时长且需要专门的仪器设备,不能满足上述检测要求.近年来,生物传感器作为一种有效的替代方法已广泛应用于抗生素检测.其中,以适配体作为分子识别元件的生物传感方法由于具有稳定性高、特异性强、信号传导多样化等优势,而备受关注.本文系统综述了现有抗生素适配体体外筛选的最新进展,并综述了基于比色、光学、电化学等信号转换模式的适配体传感器,最后对其未来在小分子污染物检测领域的应用进行了展望.Since the environmental pollution caused by antibiotic overuse and the emergence of drug-resistant bacteria have become a serious globe problem,it is of great significance to rapidly,sensitively,and accurately detect antibiotics for the preservation of ecosystems and human health.However,the traditional detection methods often need the long detection time and specialized equipment,thus they cannot meet the above detection requirement.Recently,biosensors as useful alternatives have been widely applicated in the antibiotic detection.Among them,the aptamer-based biosensing method have attracted great interest,due to its advantages of high stability,high specificity,and diverse signaling.In this paper,we thoroughly review the in vitro selection of current antibiotic aptamers.Furthermore,we highlight the recent advances of aptasensors based on colorimetric,optical,electrochemical and other signaling modes.Additionally,we offer a forecast for the development of the field of small molecule contamination detection.

关 键 词：抗生素检测 适配体 体外筛选 适配体传感器 生物传感. 

分 类 号：X-1[环境科学与工程] O6[理学—化学]