分子印迹固相萃取-同位素稀释气相色谱质谱法测定肉制品中16种欧盟优控多环芳烃  

Determination of 16 European priority polycyclic aromatic hydrocarbons in meat products by the molecularly imprinted solid phase extraction-isotope dilution-gas chromatography mass spectrometry

作　　者：鞠香 陈克云 李海霞 陈倩倩 王艳丽 梁秀清 刘艳明 汪勇 JU Xiang;CHEN Keyun;LI Haixia;CHEN Qianqian;WANG Yanli;LIANG Xiuqing;LIU Yanming;WANG Yong(Shandong Institute for Food and Drug Control,Key Laboratory of Supervising Technology for Meat and Meat Products for State Market Regulation,Industrial Technology Foundation Public Service Platform,Jinan,250101,China;Shimadzu Enterprise Management(China)Co.,LTD.,Shanghai,210005,China)

机构地区：[1]山东省食品药品检验研究院,国家市场监管重点实验室(肉及肉制品监管技术),产业技术基础公共服务平台,济南250101 [2]岛津企业管理(中国)有限公司,上海210005

出　　处：《环境化学》2024年第8期2864-2868,共5页Environmental Chemistry

基　　金：山东省药品监督管理局科研项目(SDNMPAFZLS202301)资助.

摘　　要：结合分子印迹固相萃取对多环芳烃的特异性净化,建立了同位素稀释气相色谱质谱法测定肉制品中16种欧盟优控多环芳烃的分析方法.样品加入7种氘代同位素内标,经正己烷+二氯甲烷(7:3,V/V)混合溶液提取,分子印迹固相萃取柱净化,选择离子监测模式(SIM)下采集.在1.0—200 ng·mL^(−1)范围内,16种欧盟优控多环芳烃线性相关系数(r)均大于0.9990,不同加标浓度下,回收率为87.1%—114.8%,相对标准偏差(RSDs)(n=6)在1.0%—5.5%范围内,方法的检出限为0.2—0.3μg·kg^(−1),定量限为0.6—0.9μg·kg^(−1).方法前处理简单高效,灵敏度高,适用大批量肉制品中多环芳烃的快速测定.Based on the specific purification of the molecularly imprinted solid phase extraction,a method for the determination of 16 EU priority polycyclic aromatic hydrocarbons(PAHs)in meat products by isotope dilution-gas chromatography mass spectrometry was established.Seven deuterium isotope internal standards were added to the samples,which were extracted by a mixture of n-hexane-dichloromethane(7:3,V/V).The samples were purified by the molecular imprinting column,separated by the DB-EUPAH column,collected in select ion monitoring mode(SIM),and quantified by the internal standard method.In the range of 1.0—200 ng·mL^(−1),the linear correlation coefficients (r^(2)) were all greater than 0.9990, the average recoveries were in the range of 87.1%- 114.8% and the relative standard deviations (RSDs) (n=6) were ranged from 1.0% to 5.5%. The detection limits of the method were 0.2-0.3 μg·kg^(−1) and the limits of quantification were 0.6- 0.9 μg·kg^(−1) respectively. The method is simple, efficient, sensitive and suitable for the rapid determination of the EU PAHs in meat products.

关 键 词：欧盟优控多环芳烃 气相色谱质谱法 同位素稀释 分子印迹柱 肉制品. 

分 类 号：X-1[环境科学与工程] O6[理学—化学]