海洋小红酵母H117利用高盐有机废弃物的特性分析  

Characteristic analysis of the marine Rhodotorula minuta H117 for transformation of high salt organic wastes

作　　者：温瑞玲 仉倩 程仕伟[1] 陈卫卫 曲冠云 王新杰 葛宜和[1] WEN Ruiling;ZHANG Qian;CHENG Shiwei;CHEN Weiwei;QU Guanyun;WANG Xinjie;GE Yihe(Yantai Engineering Laboratory of Biological Fermentation and Separation,School of Life Sciences,Ludong University,Yantai 264025,China;Shandong Seasir Brewing Food Co.,Ltd.,Yantai 261411,China)

机构地区：[1]鲁东大学生命科学学院,烟台市生物发酵与分离工程实验室,山东烟台264025 [2]山东食圣酿造食品有限公司,山东烟台261411

出　　处：《中国渔业质量与标准》2024年第3期1-7,共7页Chinese Fishery Quality and Standards

基　　金：烟台市科技计划发展项目(2022XDRH029);山东省自然科学基金项目(ZR2019MC037,ZR2020MC012);山东省大学生创新创业训练计划项目(S202110451091)。

摘　　要：高盐有机废弃物属于难处理的污染物,为探究利用微生物处理高盐有机废弃物的可行性,本研究以海洋小红酵母H117为研究对象,经形态观察、生理生化、26S rRNA序列分析,确定为小红酵母(Rhodotorula minuta)。在此基础上,通过海洋小红酵母H117在高盐模拟有机废水、高盐酱油废水、高盐酱油残渣中培养后的菌落计数分析,发现其能耐受9%(m/v)盐浓度;以高盐酱油残渣为基料生长良好,最大活菌数为2.91×10^(9)cfu/mL,而在高盐酱油废水中菌落数目略少。本研究为海洋小红酵母H117转化高盐有机废弃物制备海水养殖用微生态制剂的深入研究提供了数据支撑。High-salt organic waste is a kind of pollutants that difficult to treat.In order to explore the feasibility of microbial transformation to treat high-salt organic waste,this study used the marine yeast H117as the research object,and Rhodotorula minuta was identified through morphological observation,physiological and biochemical analysis,and 26S rRNA sequence analysis.On this basis,the colony count analysis of R.minuta H117 was conducted in the simulated high-salt organic wastewater,high-salt soy sauce wastewater and high-salt soy sauce residue,and the results showed that R.minuta H117 could tolerate 9%(m/v)salt concentration,and the maximum viable cell number was 2.91×10^(9)cfu/mL in the high-salt soy sauce residue as the base material,while the number of colonies in the high salt soy sauce wastewater was lower relatively.This study provides data support for further research on the microecological preparations for mariculture using the Rhodotorula minuta H117 for transformation of high salt organic wastes.

关 键 词：小红酵母 耐盐 工业废水 微生物转化 

分 类 号：Q815[生物学—生物工程]