钼酸铵分光光度法测定渔业水体中总磷含量的测量不确定度评定  

Measurement uncertainty assessment of ammonium molybdate spectrophotometric determination of total phosphorus content in fishery water

作　　者：刘祝萍 陈家长[1,2] 宋超 裘丽萍[1,2] 李丹丹 方龙香[1,2] 范立民 孟顺龙[1,2] LIU Zhuping;CHEN Jiazhang;SONG Chao;QIU Liping;LI Dandan;FANG Longxiang;FAN Limin;MENG Shunlong(Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China;Ministry of Agriculture and Rural Affairs:risks of aquatic product quality,safety and environmental factors Evaluation Laboratory(Wuxi),Wuxi 214081,China)

机构地区：[1]中国水产科学研究院淡水渔业研究中心,江苏无锡214081 [2]农业农村部水产品质量安全环境因子风险评估实验室(无锡),江苏无锡214081

出　　处：《中国渔业质量与标准》2024年第3期8-15,共8页Chinese Fishery Quality and Standards

基　　金：国家特色淡水鱼产业技术体系(CARS-46);中国水产科学研究院中央级公益性科研院所基本科研业务费专项(2023TD18)。

摘　　要：总磷作为评价水体富营养化的主要指标之一,是水产养殖过程中水质监测的重要检测指标。为了测定渔业水体中总磷含量的不确定度,依据GB 11893—1989《水质总磷的测定钼酸铵分光光度法》测量渔业水体中总磷含量,依据JJF 1059.1—2012《测量不确定度评定与表示》评定总磷含量的测量不确定度。通过建立测量模型,分析钼酸铵光度法在总磷测定过程中不确定度主要来源可分为3大类:样品重复测量引入的不确定度、试样在测定磷含量过程中引入的不确定度(包括磷标准贮备溶液引入的标准不确定度、磷标准使用溶液配制过程引入的不确定度、总磷标准曲线测试点移取过程引入的不确定度、总磷标准曲线拟合引入的不确定度和检测仪器测定总磷精密度引入的不确定度)以及样品在取样、消解、定容过程引入的不确定度。试样中总磷含量的测量结果可以报告为:C=(0.19±0.01)mg/L(k=2)。Total phosphorus,as one of the main indicators for evaluating water eutrophication,is an important detection indicator for water quality monitoring in aquaculture processes.In order to determine the uncertainty of total phosphorus content in fishery water bodies,the total phosphorus content in fishery water bodies was measured according to GB 11893—1989“Determination of total phosphorus in water quality-Ammonium molybdate spectrophotometric method”,and the measurement uncertainty of total phosphorus content was evaluated according to JJF 1059.1—2012"Evaluation and Expression of Measurement Uncertainty".By establishing a measurement model,the main sources of uncertainty in the determination of total phosphorus by ammonium molybdate photometry can be divided into three categories:uncertainty introduced by repeated sample measurements The uncertainty introduced by the sample in the process of determining phosphorus content(including the standard uncertainty introduced by the phosphorus standard storage solution,the uncertainty introduced by the phosphorus standard solution preparation process,the uncertainty introduced by the total phosphorus standard curve testing point moving process,the uncertainty introduced by the total phosphorus standard curve fitting,and the uncertainty introduced by the detection instrument in determining the total phosphorus precision),as well as the uncertainty introduced by the sample in sampling,digestion The uncertainty introduced by the constant volume process.The expanded uncertainty of total phosphorus content in the sample can be expressed as:C=(0.19±0.01)mg/L;k=2.

关 键 词：总磷含量 钼酸铵分光光度法 渔业水体 测量不确定度 评定 

分 类 号：S949[农业科学—水产养殖]