蚕豆根尖细胞微核检测方案与数据分析  

Micronucleus detection design and data analysis of Vicia faba root-tip cells

作　　者：李雅轩[1] 张飞雄[1] 赵昕[1] 胡英考[1] 晏月明[1] 李小辉[1] LI Yaxuan;ZHANG Feixiong;ZHAO Xin;HU Yingkao;YAN Yueming;LI Xiaohui(College of Life Sciences,Capital Normal University,Beijing 100048)

机构地区：[1]首都师范大学生命科学学院,北京100048

出　　处：《首都师范大学学报（自然科学版）》2024年第4期85-89,共5页Journal of Capital Normal University：Natural Science Edition

基　　金：北京市自然科学基金项目(6212001)。

摘　　要：微核检测技术在世界范围内被广泛应用于环境监测以及食品安全性检测,由于其可操作性强,亦是大学开展课外活动,培养学生科研能力的有效方法。本文针对传统方法中本底微核数变异大和检验效率低的问题,提出了新的设计方案及分析方法。对师生进行课外研究及环境因素的遗传安全性分析与鉴定具有一定指导意义。Micronucleus detection technology is widely used in environment monitoring and food safety inspecting all over the world and it is also a routine method for undergraduates to perform scientific research thinking training in genetics experiment teaching of university.In this study,a proposal was presented to enhance the test efficiency of micronucleus detection technology.Compared with the traditional micronucleus detection method,the new method is easier to find the difference among the experimental data and therefore identify the disadvantageous effectors on biological development.This work has a certain guiding significance for teachers and students to carry out the research of laboratory opening project and identification of genetic safety of environmental factors.

关 键 词：微核 微核检测 有丝分裂指数 数据分析 生物统计 

分 类 号：Q3-3[生物学—遗传学]