一例简单的席夫碱探针对次氯酸根的荧光开启识别及生物成像应用  

A simple Schiff base probe for the fluorescent turn⁃on detection of hypochlorite and its biological imaging application

作　　者：闫金龙[1] 吴伟娜 王元 YAN Jinlong;WU Weina;WANG Yuan(College of Science and Technology,Jiaozuo Normal College,Jiaozuo,Henan 454001,China;College of Chemistry and Chemical Engineering,Henan Key Laboratory of Coal Green Conversion,Henan Polytechnic University,Jiaozuo,Henan 454000,China)

机构地区：[1]焦作师范高等专科学校理工学院,焦作454001 [2]河南理工大学化学化工学院,河南省煤炭绿色转化重点实验室,焦作454000

出　　处：《无机化学学报》2024年第9期1653-1660,共8页Chinese Journal of Inorganic Chemistry

基　　金：河南省自然科学基金(No.242300420190);河南省科技攻关项目(No.242102320058);河南省教育厅高等学校重点科研项目(No.24A150010);河南省高校基本科研业务费项目(No.NSFRF240809,NSFRF230402);河南理工大学“双一流”创建工程项目(No.AQ20230745,AQ20230754);焦作师范高等专科学校高层次科研培育基金项目(No.GJ-2023-06)资助。

摘　　要：通过缩合反应制备了一例简单的席夫碱探针苯并色烯-2-甲醛缩二氨基马来腈(1),使用核磁共振氢谱/碳谱、质谱和单晶X射线衍射等手段表征了探针的结构。荧光测试表明,探针1自身无荧光,而次氯酸根(ClO^(-))能够特异性打开探针1在530 nm处的强荧光发射。探针1对ClO^(-)响应灵敏且在数秒内完成。通过质谱和理论计算手段研究了ClO^(-)介导的探针1的分解反应机理。此外,该探针还可用于活细胞、斑马鱼和拟南芥中ClO^(-)的荧光成像。A simple Schiff base probe,namely 2-(((E)-(3H-benzo[f]chromen-2-yl)methylene)amino)-3-aminomaleon-itrile(1),was prepared by the condensation reaction,which was characterized through 1H NMR,13C NMR,ESI-MS,and single crystal X-ray diffraction.The fluorescence experiments indicated that probe 1 was non-emissive,while hypochlorite(ClO^(-))could induce a strong emission of the probe at 530 nm.The response of probe 1 toward ClO^(-)was highly sensitive and finished within a few seconds.The ClO^(-)-promoted decomposition of probe 1 was suggested by spectral and theoretical methods.Furthermore,the probe was applied to imaging exogenous and endogenous ClO^(-)in living cells,zebrafish,and Arabidopsis thaliana.CCDC:2353078.

关 键 词：苯并色烯 生物成像 荧光探针 次氯酸根 席夫碱 

分 类 号：O613.42[理学—无机化学]