陆地棉4-香豆酸辅酶A连接酶基因Gh4CL30的功能分析  

Functional analysis of 4-coumarate:CoA ligase gene Gh4CL30 in upland cotton

作　　者：巩隽铭 熊显鹏[1] 张彩霞 邵东南 程帅帅 孙杰[1] GONG Junming;XIONG Xianpeng;ZHANG Caixia;SHAO Dongnan;CHENG Shuaishuai;SUN Jie(Key Laboratory of Oasis Eco-Agriculture of Xinjiang Production and Construction Corps/Agricultural College of Shihezi University,Shihezi Xinjiang 832003,China)

机构地区：[1]石河子大学农学院/新疆生产建设兵团绿洲生态农业重点实验室,新疆石河子832003

出　　处：《新疆农业科学》2024年第6期1301-1309,共9页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(31960438);新疆生产建设兵团国际科技合作项目(2021BC001);石河子大学高层次人才科研启动项目(RCZK2021B24);石河子市重点领域科技攻关项目(2022NY01)。

摘　　要：【目的】研究4-香豆酸辅酶A连接酶(4CL)家族基因Gh4CL30的生物学功能,为棉花株型育种提供理论依据和基因种质资源。【方法】利用病毒诱导的基因沉默技术和基因编辑技术获得Gh4CL30沉默和编辑植株,测定该基因抑制及敲除植株的黄酮和木质素含量,调查棉花田间表型性状、种子大小和纤维品质。【结果】Gh4CL30基因沉默和敲除植株中木质素合成相关基因表达量显著下降,4-香豆酸辅酶A连接酶含量显著减少,茎秆中木质素含量显著降低,其株高、种子大小和纤维长度显著降低。【结论】Gh4CL30通过调控棉花木质素生物合成功能影响其生长发育。【Objective】To preliminarily study the biological function of 4-coumarate-CoA ligase(4CL)family gene gh4cl30,which might provide theoretical basis and genetic resources for cotton plant breeding.【Methods】Virus-induced gene silencing technology and gene editing technology was used to obtain gh4cl30-silenced and edited plants and determine the flavone and lignin contents of the gene suppressed and knockout plants to investigate field phenotype,seed size and fiber quality.【Results】Both gene silencing and gene editing of the gene resulted in dwarfing of the plants and a significant reduction of lignin content in the plant stalks.Field phenotypic measurements showed that seed size and fiber length were significantly reduced in the gene edited plants.【Conclusion】Gh4CL30 affects cotton growth and development mainly through regulating lignin biosynthesis.

关 键 词：陆地棉 4-香豆酸辅酶A连接酶 木质素 基因编辑 株高 

分 类 号：S188[农业科学—农业基础科学]