Identification of 3-ketocapnine reductase activity within the human microbiota  

作　　者：Xiaotong Wu Lukuan Hou Haili Zhang Yi Ma Jufang Wang Mingwei Cai Xiaoyu Tang 

机构地区：[1]School of Biology and Biological Engineering,South China University of Technology,Guangzhou,China [2]Institute of Chemical Biology,Shenzhen Bay Laboratory,Shenzhen,China [3]College of Chemistry and Pharmacy,Northwest A&F University,Yangling,China

出　　处：《mLife》2024年第2期307-316,共10页微生物（英文）

基　　金：supported by the Shenzhen Bay Laboratory Startup Funds(21230051 to X.T.);Guangdong Province's Pearl River Recruitment Program of Talents(2021QN02Y855 to X.T.);GuangDong Basic and Applied Basic Research Foundation(2021A1515110334 to M.C.);the Natural Science Basic Research Plan in Shaanxi Province of China(2023-JC-QN-0160 to L.H.);the Scientific Research Foundation of Northwest A&F University(Z1090122052 to L.H.).

摘　　要：The microbial synthesis of sulfonolipids within the human body is likely involved in maintaining human health or causing diseases.However,the enzymes responsible for their biosynthesis remain largely unknown.In this study,we identified and verified the role of 3-ketocapnine reductase,the third-step enzyme,in the four-step conversion of L-phosphoserine into sulfobacin B both in vivo and in vitro.This finding builds upon our previous research into sulfonolipid biosynthesis,which focused on the vaginal bacterium Chryseobacterium gleum DSM 16776 and the gut bacterium Alistipes finegoldii DSM 17242.Through comprehensive gene mapping,we demonstrate the widespread presence of potential sulfonolipid biosynthetic genes across diverse bacterial species inhabiting various regions of the human body.These findings shed light on the prevalence of sulfonolipid-like metabolites within the human microbiota,suggesting a potential role for these lipid molecules in influencing the intricate biointeractions within the complex microbial ecosystem of the human body.

关 键 词：3-ketocapnine reductase capnines human microbiota sulfonolipids 

分 类 号：R37[医药卫生—病原生物学]