Isolation,characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere of Acacia mangium at sandy BRIS soil  

作　　者：Zakiah MUSTAPHA Radziah OTHMAN Nik Nurnaeimah NIK MUHAMMAD NASIR Dhiya Dalila ZAWAWI Mohd Khairi CHE LAH Hafizan JUAHIR 

机构地区：[1]School of Agriculture Science and Biotechnology,Faculty of Bioresources and Food Industry,Universiti Sultan Zainal Abidin,Besut Campus,22200 Besut,Terengganu,Malaysia [2]Department of Land Management,Faculty of Agriculture,Universiti Putra Malaysia,43400 Serdang,Selangor,Malaysia [3]Faculty of Plantation and Agrotechnology,Universiti Technologi MARA Cawangan Pahang,26400 Jengka,Pahang,Malaysia

出　　处：《Frontiers of Agricultural Science and Engineering》2024年第3期499-513,共15页农业科学与工程前沿（英文版）

基　　金：supported by Ministry of Higher Education Malaysia for the Knowledge Transfer Program—KTP Community grant[KTP/Bil 003/16(KTP-R5)];Center for Research Excellence and Incubation Management of UniSZA for the Pre-Commercialization grant(UniSZA/16/DPP/RR217).

摘　　要：This study has isolated,characterized,and identified potential plant growthpromoting rhizobacteria(PGPR)with multiple PGP characteristics(N_(2)-fixation,P-and K-solubilization,IAA,and siderophores production)from the rhizosphere BRIS soil of Acacia mangium.A total of 24 pure colonies were isolated and only 8 colonies were selected for further evaluation of the growth rate in 5%organic molasses medium supplemented with 2%KNO_(3).Based on the biochemical,potential PGP characteristics and growth performance,3 superior PGPR strains were selected and identified as Paraburkholderia unamae(UA1),Bacillus amyloliquefaciens(UA6),and Enterobacter asburiae(UAA2)by partial sequencing of the 16S rRNA gene.The selected bacterial strains either in single or mixed(UA1+UA6+UAA2)cultures have shown a significant biochemical estimation of the PGP characteristics.Each strain has its own PGPR traits superiority with UA1 showing the best PGP characteristic followed by UA6 and UAA2.The use of mixed bacterial strains was beneficial as it showed the best performance in N_(2)-fixation,siderophores production,and significant effect on corn phenology,growth and yield compared to using a single strain.These types of microbes showed potential to be used as biofertilizer and should be exploited more.

关 键 词：BIOFERTILIZER corn organic molasses medium PGP characteristic PHYTOHORMONE potassium nitrate SIDEROPHORES 

分 类 号：S154.3[农业科学—土壤学]