高效液相色谱法测定香苏理气止痛颗粒剂中橙皮苷的含量  

Determination of Hesperidin in Xiangsuliqizhitong Granules by HPLC

作　　者：于辉 孙春艳 胡冬梅 吴瑞 高翠芳 高秀蕊 张霄汉 YU Hui;SUN Chunyan;HU Dongmei;WU Rui;GAO Cuifang;GAO Xiurui;ZHANG Xiaohan(Shandong Pharmaceutical and Food Vocational College,Zibo Shandong 255000,China)

机构地区：[1]山东药品食品职业学院,山东淄博255000

出　　处：《江西化工》2024年第4期26-28,32,共4页Jiangxi Chemical Industry

摘　　要：目的:建立测定香苏理气止痛颗粒剂中橙皮苷含量的高效液相色谱法(HPLC)。方法:以十八烷基硅烷键合硅胶(ODS柱)为填充剂,流动相为乙腈-水(18:82),流速(v)=1.0 mL/min,柱温(T)=30℃,在波长(λ)283 nm时检测。结果:橙皮苷浓度为16.3617μg/mL~81.8085μg/mL与峰面积呈线性关系,相关系数r为0.9999,回收率平均值为99.28%。结论:本研究建立的方法操作简便,结果准确,适用于香苏理气止痛颗粒剂的质量研究。Objective:To establish a HPLC method for the determination of hesperidin in Xiangsuliqizhitong granules.Methods:Octadecylsilane bonded silica gel(ODS)was used as filler.The mobile phase was acetonitrile-water(18:82),the flow rate(v)was 1.0 mL/min,the column temperature(T)was 30℃,and the detection wavelength(λ)was 283nm.Results:The concentration of hesperidin in the range of 16.3617μg/mL~81.8085μg/mL showed a linear relationship with the peak area,the correlation coefficient(r)was 0.9999,and the average recovery was 99.28%.Conclusion:The method established in this study is simple and accurate,which is suitable for the quality study of Xiangsuliqizhitong granules.

关 键 词：香苏理气止痛颗粒剂 高效液相色谱法 橙皮苷 

分 类 号：R286[医药卫生—中药学]