姜黄素改善氧化鱼油对黄颡鱼肠道的不利影响  

Curcumin Improves Adverse Effects of Oxidized Fish Oil on Intestine of Pelteobagrus fulvidraco

作　　者：陈严轩 王国霞 邱建强 刘燕 史合群 董芮绮 彭凯 CHEN Yanxuan;WANG Guoxia;QIU Jianqiang;LIU Yan;SHI Hequn;DONG Ruiqi;PENG Kai(Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition,Ministry of Agriculture and Rural Affairs,Key Laboratory of Animal Nutrition and Feed Science in South China,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;College of Animal Science and Technology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China;Guangzhou Cohoo Biotechnology Co.,Ltd.,Guangzhou 510145,China;College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]广东省农业科学院动物科学研究所,农业农村部华南动物营养与饲料重点实验室,广东省畜禽育种与营养研究重点实验室,广州510640 [2]仲恺农业工程学院动物科技学院,广州510225 [3]华中农业大学水产学院,武汉430070 [4]广州市科虎生物技术有限公司,广州510145 [5]上海海洋大学水产与生命学院,上海201306

出　　处：《动物营养学报》2024年第8期5297-5307,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：广东省现代农业产业技术体系创新团队建设项目(2023KJ115);企业委托项目“姜黄素对黄颡鱼生长、免疫力、体色和肠道健康的影响”(2020横024)。

摘　　要：本试验旨在研究姜黄素对饲喂含氧化鱼油饲料的黄颡鱼(Pelteobagrus fulvidraco)肠道消化酶活性、组织形态及菌群结构的影响。设计6种等氮等脂试验饲料,饲料中分别添加3%新鲜鱼油(T1组)、3%氧化鱼油(T2组)、3%氧化鱼油+0.02%姜黄素(T3组)、3%氧化鱼油+0.04%姜黄素(T4组)、3%氧化鱼油+0.06%姜黄素(T5组)、3%氧化鱼油+0.08%姜黄素(T6组)。选取初始体重为(19.6±0.3)g的健康黄颡鱼720尾,随机分为6组,分别饲喂上述6种试验饲料,每组4个重复,每个重复30尾鱼。养殖周期为56 d。结果显示:与T1组相比,T2组肠道消化酶活性无显著差异(P>0.05),但肠道绒毛长度显著降低(P<0.05);在属水平下,T2组假单胞菌属(Pseudomonas)和罗尔斯通氏菌属(Ralstonia)的相对丰度显著下降(P<0.05),鲸杆菌属(Cetobacterium)的相对丰度显著增加(P<0.05)。与T2组相比,T6组肠道胰蛋白酶活性、绒毛宽度、杯状细胞数显著增加(P<0.05),T4~T6组肠道脂肪酶活性显著增加(P<0.05),T3~T6组肠道绒毛长度显著增加(P>0.05),T3和T6组肠道肌层厚度显著增加(P<0.05);在属水平下,T4组梭状芽孢杆菌属(Clostridium)和分节丝状菌属(Candidatus_Arthromitus)的相对丰度显著增加(P<0.05),T4~T6组Pseudomonas的相对丰度显著增加(P<0.05),Cetobacterium的相对丰度显著降低(P<0.05),T5和T6组Ralstonia的相对丰度显著增加(P<0.05)。随着饲料中姜黄素添加量的增加,肠道脂肪酶活性呈线性效应(P=0.001),绒毛长度呈二次效应(P<0.001),绒毛宽度呈线性(P=0.001)和二次效应(P<0.001),杯状细胞数呈线性(P=0.030)和二次效应(P=0.007);属水平上,Pseudomonas的相对丰度呈线性(P<0.001)和二次效应(P<0.001),Cetobacterium的相对丰度呈线性(P=0.001)和二次效应(P=0.001),Ralstonia的相对丰度呈二次效应(P<0.001)。综上可知,饲料中添加3%氧化鱼油诱导了黄颡鱼肠道组织结构损伤,而添加0.04%~0.08%姜黄素能够提高黄颡鱼肠道胰蛋白酶和脂肪酶�This study was conducted to investigate the effects of curcumin on the intestinal digestive enzyme activities,tissue morphology and microflora structure of Pelteobagrus fulvidraco fed diets containing oxidized fish oil.Six isoproteic and isolipidic diets were prepared which contained 30%fresh fish oil(T1 group),30%oxidised fish oil(T2 group),30%oxidized fish oil+0.02%curcumin(T3 group),30%oxidized fish oil+0.04%curcumin(T4 group),30%oxidized fish oil+0.06%curcumin(T5 group)and 30%oxidized fish oil+0.08%curcumin(T6 group).A total of 720 healthy Pelteobagrus fulvidraco with an initial body weight of(19.6±0.3)g were randomly distributed into six groups(four replicates in each group and 30 fish in each replicate),which were fed with each of the 6 experimental diets.The feeding trial was lasted for 56 days.The results showed as follows:compared with the T1 group,there was no significant difference in intestinal digestive enzyme activities in the T2 group(P>0.05),but the length of intestinal villi was significantly reduced(P<0.05);at the genus level,the relative abundances of Pseudomonas and Ralstonia were significantly decreased(P<0.05),and the relative abundance of Cetobacterium was significantly increased in the T2 group(P<0.05).Compared with the T2 group,the intestinal trypsin activity,villus width,goblet cell number were significantly increased in the T6 group(P<0.05),the intestinal lipase activity was significantly increased in the T4 to T6 groups(P<0.05),the intestinal villus length was significantly increased in the T3 to T6 groups(P<0.05),and the intestinal muscular thickness was significantly increased in the T3 and T6 groups(P<0.05);at the genus level,the relative abundances of Clostridium and Candidatus_Arthromitus were significantly increased in the T4 group(P<0.05),the relative abundance of Pseudomonas was significantly increased(P<0.05)and the relative abundance of Cetobacterium was significantly decreased(P<0.05)in the T4 to T6 groups,the relative abundance of Ralstonia was significantly increased in t

关 键 词：氧化鱼油 姜黄素 黄颡鱼 肠道健康 

分 类 号：S963[农业科学—水产养殖]