心肌肥大中mmu_circRNA_009396的功能研究  

Functional Study of mmu_circRNA_009396 in Cardiac Hypertrophy

作　　者：夏倩倩 曹睿琦 刘翠云[1] XIA Qian-qian;CAO Rui-qi;LIU Cui-yun(Institute for Translation Medicine,Qingdao University,Qingdao 266021,China)

机构地区：[1]青岛大学转化医学研究院,青岛266021

出　　处：《青岛大学学报（自然科学版）》2024年第3期21-26,共6页Journal of Qingdao University(Natural Science Edition)

基　　金：国家自然科学基金(批准号:81600244,82070314)资助。

摘　　要：利用血管紧张素Ⅱ(AngiotensinⅡ,AngⅡ)体外构建新生小鼠心肌细胞(Neonatal Mouse Ventricular Cardiomyocytes,NMVCs)的肥大模型,通过RT-qPCR检测肥大标志基因,采用鬼笔环肽染色心肌细胞骨架,探讨circRNA009396在心肌肥大中的调控作用。研究结果显示,1000 nmol/L AngⅡ刺激心肌细胞24 h后,circRNA009396表达水平下调,且在注射AngⅡ的小鼠心脏组织中下调。敲低circRNA009396会加重AngⅡ诱导的心肌肥大,过表达circRNA009396会抑制AngⅡ诱导的心肌肥大。这表明,circRNA009396是心肌特异性的环形RNA,能保护心脏,可能作为关键调节因子和下游信号通路共同参与调控心肌肥大过程。To investigate the regulatory role of circRNA009396 in cardiac hypertrophy,angiotensinⅡ(AngⅡ)was used to construct the hypertrophy model of neonatal mouse ventricular cardiomyocytes(NMVCs)in vitro.Hypertrophy marker genes were detected by RT-qPCR,and myocardial cytoskeleton was staining with phalloidin.The results show that the expression levels of circRNA009396 are down-regulated after 1000 nmol/L AngⅡstimulation for 24 h,and down-regulated in the heart tissue of mice injected with AngⅡ.Knockdown of circRNA009396 increases AngⅡ-induced cardiac hypertrophy,and overexpression of circRNA009396 inhibits AngⅡ-induced cardiac hypertrophy.This suggests that circRNA009396 is a cardio-specific circular RNA with cardioprotective effects,and may act as a key regulator to co-regulate the process of myocardial hypertrophy with downstream signaling pathways.

关 键 词：心肌肥大 环形RNA 血管紧张素 

分 类 号：R542.2[医药卫生—心血管疾病]