不同电荷修饰的聚苯乙烯纳米塑料对海洋微拟球藻(Nannochloropsis oceanica)生长及光合活性的影响  

Effects of Different Charged Polystyrene Nanoplastics on Growth and Photosynthetic Activity of Nannochloropsis Oceanica

作　　者：任玉东 贾志华 梁成伟 叶乃好[2,3] REN Yudong;JIA Zhihua;LIANG Chengwei;YE Naihao(College of Marine Science and Biological Engineering,Qingdao University of Science and Technology,Qingdao 266042,China;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 226071,China;Laboratory of Marine Fisheries Science and Food Production Processes,National Laboratory for Marine Science and Technology(Qingdao),Qingdao 226071,China)

机构地区：[1]青岛科技大学海洋科学与生物工程学院,山东青岛266042 [2]中国水产科学研究院黄海水产研究所,山东青岛266071 [3]青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室,山东青岛266071

出　　处：《青岛科技大学学报（自然科学版）》2024年第4期14-22,共9页Journal of Qingdao University of Science and Technology:Natural Science Edition

基　　金：国家自然科学基金项目(31770393).

摘　　要：评估了30 nm阴离子羧基修饰(-COOH)和100 nm阳离子氨基修饰(-NH_(2))的聚苯乙烯(PS)NPs及其不同浓度下(PS-COOH:0、2、5、8和10 mg·L^(-1);PS-NH_(2):0、0.2、0.3、0.4和0.5 mg·L^(-1))对海洋微藻微拟球藻(Nannochloropsis oceanica)在生长及光合活性中产生的影响。结果表明,两种NPs在培养基中均出现了聚集和电位绝对值的下降;经过羧基修饰的PS对海洋微拟球藻的生长及光合活性并未产生明显的影响,而经过氨基修饰的PS在不同浓度下对海洋微拟球藻的生长及光合活性产生了不同的影响:低浓度(0.2 mg·L^(-1))PS-NH_(2)对海洋微拟球藻生长产生促进作用,高浓度(0.4和0.5 mg·L^(-1))PS-NH_(2)对海洋微拟球藻生长产生抑制作用,海洋微拟球藻在不同浓度PS-NH_(2)胁迫下显示出了低促高抑现象;并且当海洋微拟球藻暴露于0.2 mg·L^(-1)PS-NH_(2)时,叶绿素a(Chl a)含量较对照组高,而其他处理组均低于对照组;此外,在0.2 mg·L^(-1)PS-NH_(2)处理下的海洋微拟球藻Fv/Fm值一直高于对照组,而在0.5 mg·L^(-1)高浓度的PS-NH_(2)胁迫下,海洋微拟球藻的最大光化学效率始终低于对照组,这些结果与生长数据相吻合。This study aimed to evaluate effects of 30 nm anionic carboxyl-modified(-COOH)and 100 nm cationic amino-modified(-NH_(2))polystyrene(PS)NPs at different concentrations(PS-COOH:0,2,5,8 and 10 mg·L^(-1);PS-NH_(2):0,0.2,0.3,0.4 and 0.5 mg·L^(-1))on the growth and photosynthetic activity of the marine microalga N.oceanica.The results showed that the two NPs aggregated and the absolute value of potential de-creased in the medium;PS modified with carboxyl group had no obvious effect on the growth and photocombination of N.oceanica,while PS modified with amino groups in different Dif-ferent concentrations have different effects on the growth and photosynthesis of N.oceani-ca:low concentrations(0.2 mg·L^(-1))PS-NH_(2)can promote the growth of N.oceanica,high concentrations(0.4,0.5 mg·L^(-1))PS-NH_(2)can inhibit the growth of N.oceanica,N.oceanica at different concentrations of PS-Under NH_(2)stress,it showed the phenomenon of low promotion and high inhibition;and when N.oceanica was exposed to 0.2 mg·L^(-1)PS-NH_(2),the content of chlorophyll a(Chl a)was higher than that of the control group,while the other treatment groups were lower than the control group.In addition,the Fv/Fm value of N.oceanica under 0.2 mg·L^(-1)PS-NH_(2)treatment was always higher than that of the control group,while under 0.5 mg·L^(-1)high concentration PS-NH_(2)stress,the maxi-mum photochemical value of N.oceanica Efficiency was consistently lower than in the con-trol group,and these results were consistent with the growth data.

关 键 词：表面电荷 纳米塑料 聚苯乙烯(PS) 海洋微拟球藻 低促高抑 毒理效应 

分 类 号：Q89[生物学]