中药复方制剂对奶牛乳房炎主要病原菌体外抑菌活性及治疗效果评价  

Evaluation of Antibacterial Activity and Therapeutic Effect of Chinese Medicinal Prescription against Main Pathogens Causing Mastitis in Dairy Cows

作　　者：刘伯承[1] 周望平[1] 杨俊[1] 陈一峰 高帅[1] 刘海林[1] LIU Bo-cheng;ZHOU Wang-ping;YANG Jun;CHEN Yi-feng;GAO Shuai;LIU Hai-ling(Hunan Institute of Animal and Veterinary Science,Changsha 410131)

机构地区：[1]湖南省畜牧兽医研究所,长沙410131

出　　处：《中国奶牛》2024年第8期23-28,共6页China Dairy Cattle

基　　金：“十三五”国家重点研发计划(2018YFD050170);长沙市重点科技计划项目(kh20201218)。

摘　　要：为探究中药复方制剂对奶牛乳房炎病原菌抑菌活性、临床疗效及免疫功能开展了本试验。根据中医辨证理论,将蒲公英、紫花地丁、金银花、丝瓜络等15味中草药自拟4个复方,以临床型奶牛乳房炎样品分离鉴定的金黄色葡萄球菌、无乳链球菌及大肠杆菌为受试株进行体外抑菌试验。将30头患病奶牛分成3个组,选用抑菌作用较强的复方IV进行临床治疗试验,分别为高剂量组(40mL)、中剂量组(30mL)和低剂量组(20mL),并设健康组作为阴性对照。检测乳体细胞数和乳清ALT、AST、LDH、SA及淋巴细胞刺激指数(SI)、嗜中性粒细胞吞噬力。结果表明,中药复方IV对金黄色葡萄球菌、无乳链球菌及大肠杆菌的MIC值为31.25mg/mL,抑菌活性优于复方Ⅰ、Ⅱ和Ⅲ;临床治疗试验显示,高、中、低三个剂量组对临床型奶牛乳房炎有效率分别为93.3%、85.7%、78.6%,治愈率分别为80%、78.6%、71.4%;治疗组给药7d后体细胞数、乳酶及白蛋白含量均极显著低于治疗前(P<0.01),其中高、中剂量组显著低于低剂量组(P<0.05);治疗后各组淋巴细胞刺激指数及嗜中性粒细胞吞噬率均极显著高于治疗前(P<0.01),而高剂量组嗜中性粒细胞吞噬率极显著高于低剂量组(P<0.01),显著高于中剂量组(P<0.05),与健康组比较无显著差异。综合表明,中药复方IV对奶牛乳房炎源金黄色葡萄球菌、无乳链球菌及大肠杆菌有较强的抑菌活性,临床治疗效果良好;能修复乳腺上皮细胞,降低体细胞数,提高患病奶牛免疫机能。s:To investigate the antibacterial effect,clinical efficacy and immune function of Chinese herbal compound preparations against the pathogenic bacteria of mastitis in dairy cows.The trial was based on the theory of TCM identification,and 15 herbs,including Dandelion,Ziziphiopoglossum,Honeysuckle and Silphium,were used to formulate 4 prescriptions,Staphylococcus aureus,Streptococcus agalactiae and Escherichia coli isolated from clinical dairy cows with mastitis were used as subjects for in vitro inhibition tests.Thirty infected cows were divided into three groups,which were high-dose group(40 mL),medium-dose group(30 mL)and low-dose group(20 mL),and the healthy group was used as control,the milk somatic cell count,serum ALT,AST,LDH,SA,lymphocyte stimulation index(SI)and neutrophil phagocytosis were measured.The results showed that compound IV had a MIC value of 31.5mg/mL to against Staphylococcus aureus,Streptococcus agalactiae and E.coli,which was more effective than compound Ⅰ,Ⅱ and Ⅲ.Clinical treatment trials showed that the effective rates of the three dose groups,low,medium and high,on clinical dairy cows with mastitis were classified as 78.6,85.7 and 93.3,with cure rates of 71.4%,78.6%and 80%respectively;the somatic cell count,lactase and albumin were highly significantly lower in the treated groups after 7 days of administration compared to the pre-treatment period(P<0.01),with the high and medium dose groups being significantly lower than the low dose group(P<0.05);the lymphocyte stimulation index and neutrophil phagocytosis rate in the treatment group were significantly higher than before treatment,and the neutrophil phagocytosis rate in the high dose group was significantly higher than that in the low dose group(P<0.01)and significantly higher than that in the medium dose group(P<0.05),but there was no significant difference compared with the healthy group.The antimicrobial activity of the herbal formula IV on the Staphylococcus aureus,Streptococcus agalactiae and E.coli of mastitis in dairy cows w

关 键 词：中药复方制剂 奶牛乳房炎 体细胞数 临床疗效 

分 类 号：S858.23[农业科学—临床兽医学]