大豆GS基因家族全基因组鉴定及胁迫响应分析  

Genome-wide Identification and Stress Response Analysis of Soybean GS Gene Family

作　　者：武帅 辛燕妮 买春海 穆晓娅 王敏[1] 岳爱琴[1] 赵晋忠[3] 吴慎杰 杜维俊[1] 王利祥 WU Shuai;XIN Yan-ni;MAI Chun-hai;MU Xiao-ya;WANG Min;YUE Ai-qin;ZHAO Jin-zhong;WU Shen-jie;DU Wei-jun;WANG Li-xiang(College of Agronomy,Shanxi Agricultural University,Taigu 030801;College of Life Sciences,Shanxi Agricultural University,Taigu 030801;Department of Basic Science,Shanxi Agricultural University,Taigu 030801)

机构地区：[1]山西农业大学农学院,太谷030801 [2]山西农业大学生命科学学院,太谷030801 [3]山西农业大学基础部,太谷030801

出　　处：《生物技术通报》2024年第8期63-73,共11页Biotechnology Bulletin

基　　金：中央引导地方科技发展资金(YDZJSX2022A035);科技创新2030重大项目(2023ZD040350404);国家重点研发计划(2021YFD1600601-2);山西省种业创新联合攻关项目(2023xczx03);山西农业大学育种工程项目(YZGC096)。

摘　　要：【目的】谷氨酰胺合成酶(glutamine synthetase,GS)是氮代谢“GS-GOGAT循环”中的关键酶,研究GS在大豆中的家族成员以及对外界胁迫的响应情况。【方法】利用生物信息学方法,在大豆中全面鉴定GS基因,明确大豆GmGSs基因的位置与结构、蛋白的理化性质以及组织表达模式等,并对非生物胁迫的应答响应进行研究。【结果】从大豆中共鉴定出8个GS基因,位于8条染色体上,对应编码的氨基酸序列长度为356-432 aa。在10个保守基序中,8个GmGS都包含9个保守基序,GmGS7和GmGS8比其他成员多一个motif10保守基序。启动子顺式作用元件分析表明,GmGSs的启动子中包含丰富的光响应元件、激素响应元件和逆境胁迫响应元件。转录组数据分析表明GmGSs基因在所有组织中均有表达,其中GmGS3和GmGS4在各组织中表达量较高。荧光定量qPCR结果表明:在不同浓度的氯化铵处理后,大豆GS家族中GmGS4、GmGS5和GmGS7对低浓度铵盐处理后期响应最显著。且高盐胁迫处理后,GmGS5在根、茎、叶组织中表达量下降;GmGS7在根、茎组织中表达量上升。【结论】GS在大豆中共有8个成员,其中GmGS7在氯化铵处理和盐胁迫中均参与响应。【Objective】Glutamine synthetase(GS)is a key enzyme in the GS-GOGAT cycle of nitrogen metabolism.This work is aimed to investigate the family members of GS in soybean and the response to external stress.【Method】Based on bioinformatics methods,we comprehensively identified GS genes in soybean(Glycine max),clarified the location and structure of soybean GmGSs genes,the physicochemical properties of proteins,and the tissue expression patterns,and also studied the responses to abiotic stresses.【Result】The total of eight GS genes were identified from the soybean,located on eight chromosomes,corresponding to coding amino acid sequences with lengths ranging from 356 to 432 aa.Among the ten conserved motifs,all eight GmGS contained nine conserved motifs,and GmGS7 and GmGS8 had one more motif 10 conserved motif than other members.Analysis of promoter's cis-acting elements showed that the promoters of GmGSs contained abundant light-responsive elements,hormone-responsive elements,and adversity stress-responsive elements.Transcriptome data analysis showed that GmGSs genes were expressed in all tissues,with GmGS3 and GmGS4 having higher expression in all tissues.Fluorescence quantitative qPCR results revealed that GmGS4,GmGS5,and GmGS7 of soybean GS family responded most significantly to the late stage of low concentration ammonium treatment after different concentrations of ammonium chloride.And after high salt stress treatment,the expression of GmGS5 decreased in the root,stem and leaf tissues;while the expression of GmGS7 increased in root and stem tissues.【Conclusion】There are eight GS members in soybean,of which GmGS7 is involved in response to both ammonium chloride treatment and salt stress.

关 键 词：大豆 谷氨酰胺合成酶 生物信息学分析 氯化铵 盐胁迫 

分 类 号：Q943.2[生物学—植物学] S565.1[农业科学—作物学]