芥菜SRO基因家族全基因组鉴定与表达分析  

Genome-wide Identification and Expression Analysis of the SRO Gene Family in Brassica juncea L.

作　　者：杨巍 赵丽芬 唐兵 周麟笔 杨娟 莫传园 张宝会 李飞[1,2] 阮松林 邓英[1,2] YANG Wei;ZHAO Li-fen;TANG Bing;ZHOU Lin-bi;YANG Juan;MO Chuan-yuan;ZHANG Bao-hui;LI Fei;RUAN Song-lin;DENG Ying(Institute of Horticulture,Guizhou Academy of Agricultural Sciences,Guiyang 550006;Horticultural Engineering Technology Research Center of Guizhou,Guiyang 550006;College of Agricultural,Guizhou University,Guiyang 550025;College of Agriculture,Anshun University,Anshun 561000;Hangzhou Academy of Agricultural Sciences,Hangzhou 310024)

机构地区：[1]贵州省农业科学院园艺研究所,贵阳550006 [2]贵州省园艺工程技术研究中心,贵阳550006 [3]贵州大学农学院,贵阳550025 [4]安顺学院农学院,安顺561000 [5]杭州市农业科学研究院,杭州310024

出　　处：《生物技术通报》2024年第8期129-141,共13页Biotechnology Bulletin

基　　金：国家大宗蔬菜产业技术体系贵阳综合试验站(CARS-23-G37);贵州省科技计划项目(黔科合服企[2022]005、黔科合支撑[2022]一般086);2024年贵州省蔬菜产业技术体系(GZSCCYJSTX)。

摘　　要：【目的】SRO基因家族是植物特有的一类转录因子,在植物生长发育与胁迫响应中具有重要作用,研究芥菜SRO基因家族,为解析芥菜SRO基因功能与遗传改良提供理论依据。【方法】利用生物信息学鉴定油用芥菜与菜用芥菜基因组中的SRO基因家族成员,运用TBtools、MEGA、Cytoscape、NCBI、STRING、EggNOG等软件与数据库进行理化性质、序列特征、进化关系、调控网络等分析以及RT-qPCR分析盐胁迫下的表达模式。【结果】两种类型芥菜SRO基因家族成员数量存在差异,并分属A与B两大类,其中A类SRO蛋白含有WWE、PARP及RST结构域,而B类蛋白则缺少WWE结构域,SRO基因启动子区域含有多种非生物胁迫响应与激素响应的顺式作用元件,microRNA-BjuSRO-靶基因构建复杂调控网络,参与了细胞凋亡、根系形态建成、免疫应答、异源刺激的细胞反应、对活性氧的反应等生物学过程,油用芥菜BjuOSRO基因与甘蓝SRO基因具有较近的进化关系,RT-qPCR结果显示,在盐胁迫下BjuVA1a、BjuVA1e、BjuVA2a、BjuVA3a、BjuVB1b、BjuVA2a显著上调表达。【结论】芥菜SRO基因家族具有功能多样性,BjuVA1a、BjuVA1e、BjuVA2a、BjuVA3a、BjuVB1b、BjuVA2a与盐胁迫响应密切相关,可作为培育耐盐型芥菜新品种的候选基因。【Objective】SRO gene family is a class of plant-specific transcription factors,which plays an important role in plant growth and development and stress response.The study of the SRO gene family in Brassica juncea L.provides a theoretical basis for analyzing the function of SRO genes in B.juncea L.and its genetic improvement.【Method】Bioinformatics was used to identify members of the SRO gene family in the genomes of two types of oilseed mustard and vegetable mustard,the software and database such as TBtools,MEGA,Cytoscape,NCBI,STRING,EggNOG were used for the analysis of physicochemical properties,sequence characterization,evolutionary relationships,regulatory networks,etc.,as well as RT-qPCR to analyze the expression patterns under salt stress.【Result】There were differences in the number of members of the SRO gene family between the two types of B.juncea L.,and they belonged to two major classes.Among them,class A SRO proteins contained WWE,PARP and RST structural domains,whereas class B proteins were lack of WWE domains.The promoter region of the SRO genes contained a variety of cis-acting elements for abiotic stress response and hormone response.The microRNA-BjuSRO-target genes constructed a complex regulatory network,which was involved in apoptosis,root morphogenesis,immune response,cellular response to heterologous stimuli,and response to reactive oxygen species,and other biological processes.The RT-qPCR results showed that BjuVA1a,BjuVA1e,BjuVA2a,BjuVA3a,BjuVB1b,and BjuVA2a were significantly upregulated under salt stress.【Conclusion】The SRO gene family in the mustard has functional diversity,and BjuVA1a,BjuVA1e,BjuVA2a,BjuVA3a,BjuVB1b,and BjuVA2a are closely related to the response to salt stress,which can be used as candidate genes for breeding new salt-tolerant varieties of B.juncea L.

关 键 词：芥菜 SRO转录因子 生物信息学 MICRORNA调控 蛋白互作 GO富集分析 进化分析 基因表达分析 

分 类 号：Q943.2[生物学—植物学] S637[农业科学—蔬菜学]