金黄色葡萄球菌型乳房炎奶牛乳腺组织的lncRNA差异表达分析  

Analysis of LncRNA Differential Expression in Mammary Tissue of Cows with Staphylococcus aureus Mastitis

作　　者：周冉 王兴平 李彦霞 罗仍卓么 ZHOU Ran;WANG Xing-ping;LI Yan-xia;LUORENG Zhuo-ma(College of Animal Science and Technology,Ningxia University,Yinchuan 750021)

机构地区：[1]宁夏大学动物科技学院,银川750021

出　　处：《生物技术通报》2024年第8期320-328,共9页Biotechnology Bulletin

基　　金：国家自然科学基金项目(32060749);宁夏自然科学基金项目(2023AAC03049);国家现代农业产业技术体系(CARS-36)。

摘　　要：【目的】长链非编码RNA(lncRNA)可参与机体炎症反应,但其在奶牛乳房炎中的表达模式及分子调控机制仍不完全清楚。分析金黄色葡萄球菌(Staphylococcus aureus,S.aureus)型乳房炎奶牛乳腺组织中的差异表达lncRNA(DElncRNA)及其功能,为后续深入研究提供支撑。【方法】利用转录组测序(RNA-Seq)技术和生物信息学方法对健康奶牛和S.aureus诱导乳房炎的奶牛乳腺组织进行lncRNA测序、DElncRNA分析及GO和KEGG功能富集分析。【结果】在两组乳腺组织中共检测到1012个lncRNA。相比于健康奶牛组,S.aureus诱导组筛选到75个表达上调的和114个表达下调的lncRNA。GO和KEGG富集分析结果表明,上述DElncRNA可通过免疫相关信号通路而调节奶牛乳房炎症。进一步分析发现,DElncRNA TCONS_00042123、TCONS_00068055、TCONS_00108420和TCONS_00076361可能参与MAPK、NLR、Jak-STAT和TLR信号通路,进而调节S.aureus型奶牛乳房炎的发生与发展。【结论】在S.aureus诱导炎症的奶牛乳腺组织中获得了189个DElncRNA,可能通过潜在靶基因调控S.aureus型奶牛乳房炎的发生与发展过程。【Objective】Long non-coding RNA(lncRNA)is involved in inflammatory responses,however,their specific molecular regulatory mechanism in dairy mastitis remains unclear.In this study,differentially expressed lncRNA(DElncRNA)and its function in mammary tissues of cows with Staphylococcus aureus(S.aureus)-type mastitis were analysed to support subsequent in-depth studies.【Method】Transcriptome sequencing(RNA-Seq)technology and bioinformatics methods were used to carry out lncRNA sequencing,DElncRNA analysis,and GO and KEGG functional enrichment analysis of mammary tissues from healthy cows and cows with S.aureus-induced mastitis.【Result】A total of 1012 lncRNA are detected in the mammary tissues of both groups.Compared to the healthy dairy cows,total 75 lncRNA are up-regulated and 114 down-regulated in cows with S.aureus-induced mastitis.Further analysis reveals that DElncRNA TCONS_00042123,TCONS_00068055,TCONS_00108420 and TCONS_00076361 may be involved in the MAPK,NLR,Jak-STAT and TLR signaling pathways related to inflammation,regulating the occurrence and development of S.aureus-type mastitis in the dairy cows.【Conclusion】189 DElncRNA were obtained from mammary tissues of cows with S.aureus-induced inflammation,which may regulate the process of onset and progression of S.aureus-type cow mastitis through potential target genes.

关 键 词：奶牛 乳房炎 lncRNA 差异表达分析 金黄色葡萄球菌 

分 类 号：S858.23[农业科学—临床兽医学]