黟县黑猪微卫星遗传多样性分析  

Microsatellite analysis of genetic diversity of Yixian black pigs

作　　者：李雪婷 董林 王重龙[1] 刘林清[1] 张威 苏世广[1] 周学利[1] 周梅 LI Xueting;DONG Lin;WANG Chongong;LIU Linqing;ZHANG Wei;SU Shiguang;ZHOU Xuei;ZHOU Mei(Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,Key Laboratory of Pig Molecular Quantitative Genetics of Anhui Academy of Agricultural Sciences,Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety Engineering,Hefei 230001,China)

机构地区：[1]安徽省农业科学院畜牧兽医研究所,猪分子数量遗传学安徽省农业科学院重点实验室,畜禽产品安全工程安徽省重点实验室,合肥230001

出　　处：《上海农业学报》2024年第4期74-80,共7页Acta Agriculturae Shanghai

基　　金：安徽省财政农业种质资源保护与利用资金项目;安徽省农业科学院重点实验室项目(2021YL023);安徽省学术和技术带头人后备人选项目(2022H300);安徽省自然科学基金青年基金项目(2108085QC135);安徽省生猪产业技术体系项目(AHCYJSTX-05-12、AHCYJSTX-05-23);六安市产学研合作重大专项(2020年)。

摘　　要：选择55头黟县黑猪的血液样品,采用PCR(聚合酶链式反应)和PAGE(聚丙烯酰胺凝胶电泳)法对14个微卫星标记进行分型,分析黟县黑猪群体的遗传多样性。结果表明:在黟县黑猪群体中,14个微卫星位点共检测出171个等位基因,平均每个位点约12个,有效等位基因数为3.11-12.37,观测杂合度范围为0.31-1;14个微卫星位点多态性含量为0.65-0.91,均具有高度多态性,累积排除概率达99.9%,说明选择的14个微卫星标记能够对黟县黑猪的亲缘关系进行鉴定。The blood samples of 55 Yixian black pigs were selected in this experiment,and 14 microsatellite markers were typed by PCR(Polymerase chain reaction)and PAGE(Polyacrylamide gel electrophoresis)to analyze the genetic diversity of Yixian black pig population.The results showed that a total of 171 alleles were detected at 14 microsatellite loci in Yixian black pig population,with an average of about 12 alleles per locus.The number of effective alleles was 3.1112.37,and the observed heterozygosity ranged from 0.31 to 1.The polymorphism content of 14 microsatellite loci was 0.65—0.91,all of which were highly polymorphic,and the cumulative exclusion probability was 99.9%,which indicated that the selected 14 microsatellite markers could identify the genetic relationship of Yixian black pigs.

关 键 词：黟县黑猪 微卫星标记 遗传多样性 聚丙烯酰胺凝胶电泳 

分 类 号：S828[农业科学—畜牧学]