射干麻黄汤与定喘汤治疗支气管哮喘寒热证候的药理学分析  

Pharmacological of Shegan Mahuang decoction and Dingchuan decoction in the treatment of cold and heat syndrome of bronchial asthma

作　　者：陈丽平[1,2] 韩立[1] 卞华[1] 庞立业 CHEN Li-ping;HAN Li;BIAN Hua;PANG Li-ye(Henan Key Laboratory of Zhang Zhongjing Formulaeand Herbs for Immuoregulation,Nanyang Institute of Technology,Nanyang 473004,Henan Province,China;Respiratory Disease Prevention and Control of Provincial Department of Traditional Chinese Medicine to Build Collaborative Innovation Center,Henan Key Laboratory of Chinese Medicine for Respiratory Disease,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,Henan Province,China)

机构地区：[1]南阳理工学院河南省张仲景方药与免疫调节重点实验室,河南南阳473004 [2]河南中医药大学呼吸疾病中医药防治省部共建协同创新中心、河南省中医药防治呼吸病重点实验室,河南郑州450046

出　　处：《中国临床药理学杂志》2024年第16期2413-2417,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金青年科学基金资助项目(81704200);河南省自然科学基金资助项目(232300421192);河南省科技攻关基金资助项目(222102310551);第五批全国中医临床优秀人才研修基金资助项目(国中医药人教函[2022]239号)。

摘　　要：目的分析射干麻黄汤与定喘汤治疗支气管哮喘寒热证候的机制差异。方法用中药系统药理学数据库与分析平台分别筛选射干麻黄汤与定喘汤中各组成药物的有效成分及作用靶点。通过基因与疾病关联数据库和人类基因信息数据库获取支气管哮喘对应的基因靶点,分别筛选射干麻黄汤与冷哮、定喘汤与热哮的核心作用靶点及成分,并用网络分析在线平台进行网络拓扑分析,通过在R软件中用“org.Hs.eg.db”软件包进行基因本体(GO)与京都基因组百科全书(KEGG)功能富集分析,预测其不同作用机制,并用Autodock Vina软件对核心活性成分和核心靶点进行分子对接技术分析验证。结果射干麻黄汤药物活性成分130个,定喘汤药物活性成分236个,相同活性成分54个。在与支气管哮喘相关靶点中,射干麻黄汤有52个潜在治疗靶点,定喘汤有45个潜在靶点。GO与KEGG分析显示:射干麻黄汤与定喘汤在血小板活化、脂肪细胞因子信号通路、酒精性肝病、瞬时受体电位通道的炎症介质调节、炎症性肠病、心肌细胞中的肾上腺素能信号、磷脂酰肌醇3激酶-蛋白激酶B等信号通路等存在差异。射干麻黄汤中核心化合物鸢尾甲黄素9CI、鸢尾甲黄素A与定喘汤中核心化合物花生四烯酸、甘草查尔酮A等存在差异;定喘汤治疗热哮与射干麻黄汤治疗冷哮药物作用机制在信号转导与转录活化因子3(STAT3)、辣椒素受体(TRPV1)与Ras同源基因(RHO)靶点存在差异。结论2种中药方剂调节哮喘寒热证候引起的不同分子和生物过程反映了冷哮和热哮2种中医证候之间的生物学差异。Objective To analyze the difference in mechanism between Shegan Mahuang decoction and Dingchuan decoction in treating cold and heat syndrome of bronchial asthma.Methods Use the traditional Chinese medicine system pharmacology database and analysis platform to screen the effective ingredients and targets of each component of Shegan Mahuang decoction and Dingchuan decoction,respectively.Obtain gene targets corresponding to bronchial asthma through gene disease association databases and human gene information databases,screen the core targets and components of Shegan Mahuang decoction and Cold Asthma,Dingchuan decoction and Heat Asthma,and perform network topology analysis using online network analysis platforms.Perform gene ontology(GO)and Kyoto Encyclopedia of Genomes(KEGG)functional enrichment analysis using the"org.Hs.eg.db"software package in R software,predict their different mechanisms of action,and use Autodock Vina software to analyze and verify the molecular docking technology of core active ingredients and core targets.Results There are 130 active ingredients in Shegan Mahuang decoction,236 active ingredients in Dingchuan decoction,and 54 identical active ingredients.Among the targets related to bronchial asthma,Shegan Mahuang decoction has 52 potential therapeutic targets,and Dingchuan decoction has 45 potential therapeutic targets.GO and KEGG analysis showed that there were differences between Shegan Mahuang decoction and Dingchuan decoction in platelet activation,adipocyte cytokine signaling pathways,alcoholic liver disease,inflammatory mediator regulation of transient receptor potential channels,inflammatory bowel disease,adrenergic signaling in myocardial cells,phosphoinositide-3-kinase-protein kinase B signaling pathways,etc.There are differences in core compounds such as iristectorigenin(9CI)and iristectorigenin A in Shegan Mahuang decoction compared to core compounds such as arachidonic acid and glycyrrhetinic acid in Dingchuan decoction;the mechanism of action of Dingchuan decoction in treating h

关 键 词：射干麻黄汤 定喘汤 冷哮 热哮 网络药理学 分子对接技术 

分 类 号：R28[医药卫生—中药学]