基于质谱成像分析黄芩切片主要代谢产物空间分布  

Spatial Distribution of the Main Metabolites in Radix Scutellariae Slices Based on Mass Spectrometry Imaging

作　　者：肖雪 关孝康 徐周毅 陆桥 XIAO Xue;GUAN Xiao-kang;XU Zhou-yi;LU Qiao(Department of Laboratory Medicine,Taihe Hospital,Hu-bei University of Medicine,Shiyan,Hubei 442000;Hubei Key Laboratory of Wudang Local Chinese Medicine Research,Hu-bei University of Medicine,Shiyan,Hubei 442000;Department of Chemistry,College of Chemistry and Chemical Engineer-ing,Xiamen University,Xiamen,Fujian 361005;Pen-Tung Sah Institute of Micro-Nano Science&Technology,Xiamen University,Xiamen,Fujian 361005,China)

机构地区：[1]湖北医药学院附属太和医院检验部,湖北十堰442000 [2]湖北医药学院武当特色中药研究湖北省重点实验室,湖北十堰442000 [3]厦门大学化学化工学院化学系,福建厦门361005 [4]厦门大学萨本栋微米纳米科学技术研究院,福建厦门361005

出　　处：《湖北医药学院学报》2024年第4期385-389,共5页Journal of Hubei University of Medicine

基　　金：国家自然科学基金青年项目(22304050);湖北省自然科学基金青年项目(2023AFB271);湖北医药学院人才启动基金计划项目(2022QDJZR007);武当特色中药研究湖北省重点实验室(湖北医药学院)开放课题(WDCM2023021)。

摘　　要：目的:研究中药黄芩的主要代谢产物在其根部切片中的空间分布。方法:通过构建基于激光解吸等离子体电离的大气压质谱成像技术,对黄芩切片中的代谢物进行质谱成像分析。结果:蒽醌类产物分布相似,它们大部分集中在根表皮,也有一部分集中在维管柱边缘。黄芩的主要活性成分黄芩素几乎分布于整个切片中。结论:本研究证明质谱成像技术能够实现对中药材切片中内源性分子的空间分布分析,为中药材种植和加工提供了新的研究技术。Objective To investigate the spatial distribution of the main metabolites in Radix Scutellariae slices.Methods An atmospheric pressure mass spectrometry imaging was constructed based on laser desorption ionization,to analyze the the main metabolites in Radix Scutellariae slices.Results Anthraquinone products had similar distribution,with the majority being concentrated in the root epidermis and some in the edge of the vascular column.Additionally,baicalin,the main ac⁃tive ingredient of Radix Scutellariae,was found to be distributed throughout the entire section.Conclusion This study dem⁃onstrates that mass spectrometry imaging can be used to analyze the spatial distribution of endogenous molecules in Chinese medicine slices,which provides a new research tool for the cultivation and processing of these medicines.

关 键 词：黄芩 代谢产物 质谱成像 

分 类 号：R284.1[医药卫生—中药学]