连翘提取物与抗生素的体外协同对分枝杆菌的抑制作用  

Synergistic antibacterial effect of Forsythia suspensa(Thunb.)Vahl.combined with antibiotics towards mycobacteria

作　　者：李武[1,2] 敬帅 LI Wu;JING Shuai(College of Life Sciences,Neijiang Normal University,Neijiang,Sichuan 641100,China;Key Laboratory of Regional Characteristic Agricultural Resources,Neijiang Normal University,Neijiang,Sichuan 641100,China)

机构地区：[1]内江师范学院生命科学学院,四川内江641100 [2]内江师范学院、四川省高等学校特色农业资源研究与利用重点实验室,四川内江641100

出　　处：《内江师范学院学报》2024年第8期68-74,共7页Journal of Neijiang Normal University

基　　金：四川省科技厅项目(2018JY0108);内江师范学院成果转化培育项目(17CZ01);内江师范学院博士科研启动项目(15B10);大学生创新创业训练计划项目(X2017013,X2019070)。

摘　　要：研究连翘提取物联合抗生素对耻垢分枝杆菌的协同抗菌作用,以期发现有效的抗菌增敏剂.通过水煮法和超声波提取法分别获得连翘水提物和醇提物后,比较二者对耻垢分枝杆菌的最小抑菌浓度.再利用有机溶剂(石油醚、二氯甲烷、乙酸乙酯和正丁醇)对连翘醇提物进行萃取,观察各有机相与抗生素的协同抗菌活性.最后利用杀菌曲线法探索连翘醇提物的二氯甲烷萃取物与抗生素的联合抗菌效果.在本实验条件下,连翘水提物没有抗分枝杆菌活性;连翘醇提物具有一定的抗分枝杆菌活性(MIC=3 mg/mL).进一步实验证明连翘醇提物及其有机溶剂萃取物与所选抗生素联用时,表现出不同程度的协同抗菌作用.连翘醇提物的二氯甲烷萃取物与抗生素混合使用可以有效提高对耻垢分枝杆菌的抗菌效果并延长抑菌时间.本文发现连翘中存在与抗生素有协同作用的抗分枝杆菌增敏剂,可为从药用植物中分离新型抗菌物质奠定一定基础.In order to identify new anti-mycobacterial natural products or new antibacterial sensitizers from medicinal plants,the anti-mycobacterial activity of crude extract of Forsythia suspensa(Thunb.)Vahl.(Fs for short)was performed.Boiled method and ultrasonic method were used to extract whole plant of Fs.Two kinds of extracts were subjected to antibacterial test in vitro by 96 microtiter plate dilution method.The ultrasonic extract of Fs was extracted by organic solvent(petroleum ether,dichloromethane,ethyl acetate and n-butanol).Finally,the antibacterial effect of dichloromethane extract of ultrasonic extract of Fs combined with antibiotics was explored by bactericidal curve method.The results showed that when the Fs extract was used alone,the saturated concentration of the Fs water extract had no antimicrobial activity,while the MIC value of the ultrasonic extract of Fs was 3 mg/mL.Further experiments proved that the alcoholic extract of Fs and its organic solvent extracts show different levels of synergistic antibacterial effects when used in combination with selected antibiotics.The dichloromethane extract of ultrasonic extract of Fs combined with antibiotics can effectively improve the antibacterial effect on mycobacteria and prolong the bacteriostatic time.There were antibacterial synergistic agents with antibiotics in Fs,which laid a certain foundation for the extraction of natural antibacterial products from medicinal plants.

关 键 词：连翘 耻垢分枝杆菌 抗菌增敏剂 

分 类 号：R285.5[医药卫生—中药学]