Solid-state NMR studies of proteins in condensed phases  

作　　者：Jiani Xiang Xialian Wu Angelo L.Chu Junxia Lu 

机构地区：[1]Key Laboratory of Industrial Fermentation(Ministry of Education),School of Life and Health Sciences,Hubei University of Technology,Wuhan,430068,China [2]Interdisciplinary Institute of NMR and Molecular Sciences,School of Chemistry and Chemical Engineering,The State Key Laboratory of Refractories and Metallurgy,Wuhan University of Science and Technology,Wuhan,430081,China [3]Thomas Jefferson High School for Science and Technology,6560 Braddock Rd,Alexandria,VA,22312,USA

出　　处：《Magnetic Resonance Letters》2024年第3期1-10,共10页磁共振快报（英文）

基　　金：supported by the National Natural Science Foundation of China(No.32171185,No.31770790);the National Key R&D Program of China(No.2017YFA0504804).

摘　　要：Some proteins perform their biological functions by changing their material states through liquid-liquid phase separation.Upon phase separation,the protein condenses into a concentrated liquid phase and sometimes into a gel phase,changing its dynamic properties and intermolecular interactions,thereby regulating cellular functions.Although the biological significance of this phenomenon has been widely recognized by researchers,there is still a lack of a comprehensive understanding of the structural and dynamic properties of the protein in the condensed phase.In this phase,molecules usually contain domains with varied dynamic properties and undergo intermediate exchanges.Magic angle spinning(MAS)solid-state NMR(SSNMR)experiments are very powerful in studying rigid protein polymers such as amyloid.The incorporation of solution-like experiments into SSNMR and the development of J-coupling based MAS SSNMR techniques extend its ability to study partially mobile segments of proteins in a condensed liquid or gel phase which are not visible by solution NMR or dipolar-coupling based SSNMR.Therefore,it has been applied in studying protein condensation and has provided very important information that is hard to obtain by other techniques.

关 键 词：Magic angle spinning(MAS)solid-state NMR(SSNMR) Protein condenses J-coupling based MAS SSNMR Liquid-liquid phase separation 

分 类 号：Q503[生物学—生物化学] O482.532[理学—固体物理]