生物催化天然产物的糖基改构与进展  

Research progress of glycosylation modification andbiocatalytic natural products

作　　者：杨谦 邢登雪 秦磊 李春[1,2,3] YANG Qian;XING Dengxue;QIN Lei;LI Chun(School of Chemistry and Chemical Engineering/State Key Laboratory Incubation Base for Green Processing of Chemical Engineering,Shihezi University,Shihezi 832003,China;Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering,Ministry of Industry and Information Technology,Institute of Biochemical Engineering,School of Chemistry and Chemical Engineering,Beijing Institute of Technology,Beijing 100081,China;Key Laboratory of Industrial Biocatalysis,Ministry of Education,Department of Chemical Engineering,Tsinghua University,Beijing 100084,China)

机构地区：[1]石河子大学化学化工学院/化工绿色过程省部共建国家重点实验室培育基地,石河子832003 [2]北京理工大学化学与化工学院生物化工研究所/医药分子科学与制剂工信部重点实验室,北京100081 [3]清华大学化学工程系工业生物催化教育部重点实验室,北京100084

出　　处：《生命的化学》2024年第7期1216-1227,共12页Chemistry of Life

基　　金：国家自然科学基金(22138006,22108154)。

摘　　要：糖基改构是一种十分重要且独特的天然产物结构修饰方式,能有效改善天然产物的水溶性、稳定性及药理活性,在自然界中由相关的糖基转移酶(glycosyltransferases,GTs)催化完成。植物天然产物的糖基改构主要通过尿苷二磷酸糖基转移酶(uridine diphosphate glycosyltransferases,UGTs)实现,然而大多数UGTs的催化活性、稳定性、底物特异性、区域选择性较低,难以满足工业催化要求。本文综述了发生糖基化天然产物的种类及其相关糖基转移酶的分类、挖掘、表征、改造,为开展提高UGTs催化效率的研究提供参考。Glycosyl modification is a very important and unique natural product structure modification method,which can effectively improve the water solubility,stability,and pharmacological activity of natural products.In nature,it is catalyzed by related glycosyltransferases(GTs).The glycosylation modification of natural plant products is mainly achieved through uridine diphosphate glycosyltransferases(UGTs).However,the catalytic activity,stability,substrate specificity,and regional selectivity of most UGTs are low,making it difficult to meet industrial catalytic requirements.This paper reviews the types of natural products that undergo glycosylation and the classification,excavation,characterization,and modification of related glycosyltransferases,providing reference for research on improving the catalytic efficiency of UGTs.

关 键 词：UDP-糖基转移酶 天然产物 糖基化修饰 生物催化 

分 类 号：TQ28[化学工程—有机化工] TQ426.97