陈旧骨骼牙齿进行复杂亲缘关系分析  

Complex genetic relationship analysis of aged bones and teeth

作　　者：王雅楠 张庆霞 王洁 Wang Yanan;Zhang Qingxi;Wang Jie(Shan Medical University,Jinzhong 030600,Shanxi,China;Forensic Judicial Appraisal Center of Beijing Public Security Bureau,Beijing 100192,China)

机构地区：[1]山西医科大学,山西晋中030600 [2]北京市公安司法鉴定中心,北京100192

出　　处：《中国法医学杂志》2024年第4期416-421,424,共7页Chinese Journal of Forensic Medicine

摘　　要：目的 通过提取陈旧骨骼、牙齿中的DNA进行复杂亲缘关系鉴定。方法 对1-4号遗骸的股骨、牙齿进行预处理,在大体系疑难检材DNA提取仪上提取,并进行定量;血样采用96道全自动工作站提取;所有样本均采用VeriFiler Plus等5种不同试剂盒进行扩增检测;对1号遗骸、孙1进行线粒体全基因组测序;根据检验结果进行亲缘关系分析。结果 根据常染色体STR分型,1号与2号遗骸存在亲缘关系,1号遗骸与贾1、贾2、贾3、孙1存在祖孙关系;根据Y染色体STR分型,2号、3号遗骸与贾1、贾2、贾3倾向来自同一父系,4号遗骸与贾1等Y-STR分型不一致,不符合同一父系遗传特征;根据mtDNA全基因组检测结果,不排除1号遗骸与孙1来自同一母系。结论 通过优化预处理、提取方法以及多种检测技术联合应用,并运用多种亲缘评判标准,能够对陈旧的骨骼、牙齿等疑难检材进行复杂亲缘分析。Objective To identify the complex relationship by extracting DNA from old bones and teeth.Methods The femurs and teeth of No.1-4 remains were pretreated,and DNA was extracted by using a large system difficult material DNA extraction instrument,and quantitated.The blood samples were extracted on the 96 fully automated workstations.all samples were amplified by using 5 different kits,such as VeriFiler Plus.The mtDNA of No.1 remains and Sun1 were sequenced.Results Based on the results of autosomal STR genotypes,the parental relationship between No.1 remains and No.2 remains was found.And grandparental relationships among No.1 remains and Jia 1,Jia 2,Jia 3 and Sun 1 were found.According to the results of Y chromosome STR genotypes,the No.2 and No.3 remains were tended to come from the same paternal lineage as Jia 1,Jia 2 and Jia 3,while the relationship of No.4 remains and Jia 1 et al,were excluded,and did not conform to the genetic characteristics of the same paternal line.Based on the results of mtDNA whole genome genotypes,it cannot be ruled out that the No.1 remains and Sun 1 come from the same maternal line.Conclusion By Optimizing the pretreatment and extraction methods,combined analysis of multi-genotyping system and multiple detection methods and based on multiple judging criteria of kinship could help to address more complex kinship tests in difficult specimens such as aged bones,teeth and so on.

关 键 词：法医物证学 复杂亲缘关系 DNA测序 陈旧骨骼 

分 类 号：D919.2[医药卫生—法医学]