Silencing of early auxin responsive genes MdGH3-2/12 reduces the resistance to Fusarium solani in apple  

作　　者：Qianwei Liu Shuo Xu Lu Jin Xi Yu Chao Yang Xiaomin Liu Zhijun Zhang Yusong Liu Chao Li Fengwang Ma 

机构地区：[1]State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple,College of Horticulture,Northwest A&F University,Yangling 712100,China

出　　处：《Journal of Integrative Agriculture》2024年第9期3012-3024,共13页农业科学学报（英文版）

基　　金：supported by the Earmarked Fund for the China Agriculture Research System(CARS-27);the Key Science and Technology Special Projects of Shaanxi Province,China(2020zdzx03-01-02).

摘　　要：Apple replant disease(ARD)has led to severe yield and quality reduction in the apple industry.Fusarium solani(F.solani)has been identified as one of the main microbial pathogens responsible for ARD.Auxin(indole-3-acetic acid,IAA),an endogenous hormone in plants,is involved in almost all plant growth and development processes and plays a role in plant immunity against pathogens.Gretchen Hagen3(GH3)is one of the early/primary auxin response genes.The aim of this study was to evaluate the function of MdGH3-2 and MdGH3-12 in the defense response of F.solani by treating MdGH3-2/12 RNAi plants with F.solani.The results show that under F.solani infection,RNAi of MdGH3-2/12 inhibited plant biomass accumulation and exacerbated root damage.After inoculation with F.solani,MdGH3-2/12 RNAi inhibited the biosynthesis of acid-amido synthetase.This led to the inhibition of free IAA combining with amino acids,resulting in excessive free IAA accumulation.This excessive free IAA altered plant tissue structure,accelerated fungal hyphal invasion,reduced the activity of antioxidant enzymes(SOD,POD and CAT),increased the reactive oxygen species(ROS)level,and reduced total chlorophyll content and photosynthetic ability,while regulating the expression of PR-related genes including PR1,PR4,PR5 and PR8.It also changed the contents of plant hormones and amino acids,and ultimately reduced the resistance to F.solani.In conclusion,these results demonstrate that MdGH3-2 and MdGH3-12 play an important role in apple tolerance to F.solani and ARD.

关 键 词：Fusarium solani early auxin responsive gene apple replant disease plant hormone antioxidant 

分 类 号：S436.611.1[农业科学—农业昆虫与害虫防治]