Cytospora pyri promotes Erwinia amylovora virulence by providing metabolites and hyphae  

作　　者：Tong Shen Mengdi Ye Yeping Xu Bohan Ding Hongtao Li Li Zhang Jun Wang Yanli Tian Baishi Hu Youfu Zhao 

机构地区：[1]College of Plant Protection/Key Laboratory of Integrated Management of Crop Diseases and Pests,Nanjing Agricultural University,Nanjing 210095,China [2]Key Laboratory of Plant Quarantine Pests Monitoring and Control,Ministry of Agriculture and Rural Affairs,Nanjing 210095,China [3]Plant Protection and Quarantine Station of Xinjiang Uygur Autonomous Region,Urumqi 830001,China [4]Xinjiang Key Laboratory of Agricultural Biosafety,Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830013,China [5]College of Agriculture,Xinjiang Agricultural University,Urumqi 830052,China [6]Plant Pathology Department,Washington State University,Prosser,WA 24106,USA

出　　处：《Journal of Integrative Agriculture》2024年第9期3045-3054,共10页农业科学学报（英文版）

基　　金：supported by the Major Science and Technology Projects in Xinjiang,China(2023A02006).

摘　　要：Bacterial-fungal interactions are widespread in nature.We observed that pear orchards affected by Cytospora pyri(formerly Valsa pyri)were often accompanied with Erwinia amylovora.However,the relationship of the two pathogens was unclear.The objective of this study was to determine whether the synergistic effect exists between E.amylovora and C.pyri.We first analyzed the coexistence frequencies of E.amylovora and C.pyri in pear trees.Virulence of the two pathogens,growth,physical interactions,amylovoran production,and expression of genes for amylovoran biosynthesis were conducted.Our results showed that E.amylovora and C.pyri could coexist on the same lesion and caused much more severe disease.We also found that E.amylovora could physically attach to C.pyri and the expression of amylovoran biosynthesis genes were up-regulated with fungal metabolite treatment.These results indicate that E.amylovora and C.pyri can cooperatively interact,which provides C.pyri with an opportunity to promote bacterial dispersal and production of virulence factor in E.amylovora.

关 键 词：pear fire blight Erwinia amylovora Valsa canker amylovoran synergy 

分 类 号：S436.612.1[农业科学—农业昆虫与害虫防治]