A unique role of the pyrimidine de novo synthesis enzyme ODCase in Lysobacter enzymogenes  

作　　者：Mingming Yang Yunxiao Tan Jiabing Ma Yingjia Zhao Xia Yan Nana Wang Pingping Wang Jiaqi Tan Suilong Ai Xiaofei Liang Bangshuai Chang Obadah E.A.Yousif Chao Zhao Bo Wang Guoliang Qian Lili Huang 

机构地区：[1]State Key Laboratory for Crop Stress Resistance and High-Efficiency Production,Northwest A&F University,Yangling 712100,China [2]College of Plant Protection,Northwest A&F University,Yangling 712100,China [3]College of Life Sciences,Northwest A&F University,Yangling 712100,China [4]College of Natural Resources and Environment,Northwest A&F University,Yangling 712100,China [5]Shaanxi Provincial Tobacco Corporation of China National Tobacco Corporation,Xi’an 710061,China [6]Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China [7]College of Plant Protection,Laboratory of Plant Immunity,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Journal of Integrative Agriculture》2024年第9期3066-3077,共12页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32102283 to Mingming Yang);the Science and Technology Major Project of China National Tobacco Corporation(110202101056(LS-16));the Science and Technology Project of Shaanxi Branch of China National Tobacco Corporation(KJ-2021-02 and KJ-2022-04).

摘　　要：Bacterial species of the genus Lysobacter are environmentally ubiquitous with strong antifungal biocontrol potential.Heat-stable antifungal factor(HSAF)secreted by the biocontrol bacterium Lysobacter enzymogenes OH11 has broad-spectrum and highly efficient antifungal activity.Studying the biosynthetic regulations of HSAF would lay an important foundation for strain engineering toward improved HSAF production.In this work,we demonstrate that Le0752,an orotidine-5´-phosphate decarboxylase enzyme(ODCase)catalyzing a pivotal step of the UMP de novo biosynthesis pathway,is vital for HSAF-mediated antimicrobial activities and growth of L.enzymogenes OH11,but not for twitching motility.This gene regulates the production of HSAF by affecting the expression of lafB,a key gene in the HSAF biosynthesis operon,through the transcription factor Clp.Interestingly,bioinformatics analysis revealed that Le0752 belongs to the Group III ODCases,whereas its homologs in the closely related genera Xanthomonas and Stenotrophomonas belong to Group I,which contains most ODCases from Gram-positive bacteria,Gram-negative bacteria and cyanobacteria.Moreover,the Group I ODCase PXO_3614 from the Xanthomonas oryzae pv.oryzae PXO99A strain complemented the Le0752 mutant in regulating HSAF-mediated antagonistic activity.Together,these results highlight the important requirement of de novo pyrimidine biosynthetic enzymes for antibiotic HSAF production in L.enzymogenes,which lays an important foundation for improving HSAF production via metabolic flow design and for dissecting the regulatory functions of bacterial ODCases.

关 键 词：ODCase pyrimidine de novo synthesis secondary metabolite HSAF Lysobacter enzymogenes 

分 类 号：S476[农业科学—农业昆虫与害虫防治]