Mitigation of N_(2)O emissions in water-saving paddy fields:Evaluating organic fertilizer substitution and microbial mechanisms  

作　　者：Delei Kong Xianduo Zhang Qidong Yu Yaguo Jin Peikun Jiang Shuang Wu Shuwei Liu Jianwen Zou 

机构地区：[1]College of Environment and Resources/College of Carbon Neutrality,Zhejiang A&F University,Hangzhou 311300,China [2]Jiangsu Key Laboratory of Low Carbon Agriculture and GHGs Mitigation,College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,China [3]Department of Biochemistry and Molecular Biology,College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China [4]Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization,Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Journal of Integrative Agriculture》2024年第9期3159-3173,共15页农业科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2022YFD2300300);the National Natural Science Foundation of China(41907072);the Scientific Research Foundation of Zhejiang A&F University,China(2022LFR003);the Jiangsu Agriculture Science and Technology Innovation Fund,China(CX(21)3007).

摘　　要：Water-saving irrigation strategies can successfully alleviate methane emissions from rice fields,but significantly stimulate nitrous oxide(N_(2)O)emissions because of variations in soil oxygen level and redox potential.However,the relationship linking soil N_(2)O emissions to nitrogen functional genes during various fertilization treatments in water-saving paddy fields has rarely been investigated.Furthermore,the mitigation potential of organic fertilizer substitution on N_(2)O emissions and the microbial mechanism in rice fields must be further elucidated.Our study examined how soil N_(2)O emissions were affected by related functional microorganisms(ammonia-oxidizing archaea(AOA),ammonia-oxidizing bacteria(AOB),nirS,nirK and nosZ)to various fertilization treatments in a rice field in southeast China over two years.In this study,three fertilization regimes were applied to rice cultivation:a no nitrogen(N)(Control),an inorganic N(Ni),and an inorganic N with partial N substitution with organic manure(N_(i)+N_(o)).Over two rice-growing seasons,cumulative N_(2)O emissions averaged 0.47,4.62 and 4.08 kg ha^(−1)for the Control,Ni and N_(i)+N_(o)treatments,respectively.In comparison to the Ni treatment,the N_(i)+N_(o)fertilization regime considerably reduced soil N_(2)O emissions by 11.6%while maintaining rice yield,with a lower N_(2)O emission factor(EF)from fertilizer N of 0.95%.Nitrogen fertilization considerably raised the AOB,nirS,nirK and nosZ gene abundances,in comparison to the Control treatment.Moreover,the substitution of organic manure for inorganic N fertilizer significantly decreased AOB and nirS gene abundances and increased nosZ gene abundance.The AOB responded to N fertilization more sensitively than the AOA.Total N_(2)O emissions significantly correlated positively with AOB and nirS gene abundances while having a negative correlation with nosZ gene abundance and the nosZ/nirS ratio across N-fertilized plots.In summary,we conclude that organic manure substitution for inorganic N fertilizer decreased so

关 键 词：organic manure substitution inorganic fertilizer N_(2)O functional microbe rice paddy 

分 类 号：S511[农业科学—作物学] S141