一测多评法同时测定花椒和竹叶花椒中4个黄酮成分  

Simultaneous determination of 4 flavonoids in Zanthoxyli Pericarpium and Pericarpium Zanthoxyli Armati by QAMS

作　　者：唐飞飞 丁昊玥 方丹纯 陈芳 张静妍 吴晓青[1] 谭睿[1] TANG Fei-fei;DING Hao-yue;FANG Dan-chun;CHEN Fang;ZHANG Jing-yan;WU Xiao-qing;TAN Rui(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China)

机构地区：[1]西南交通大学生命科学与工程学院,成都610031

出　　处：《药物分析杂志》2024年第8期1320-1330,共11页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立一测多评(QAMS)法同时测定花椒和竹叶花椒中芦丁、金丝桃苷、异槲皮苷、槲皮苷的含量,比较黄酮成分含量差异。方法:花椒甲醇提取液分析,采用Agilent Eclipse Plus C_(18)(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈(A)-0.1%甲酸水(B),梯度洗脱,体积流量1.0 mL·min^(-1),柱温35℃,检测波长360 nm,进样量10μL。以金丝桃苷为内标,计算其他3个成分的相对校正因子,测定花椒和竹叶花椒中4个黄酮成分的含量。结果:4个成分在各自范围内线性关系良好(r≥0.9999),在花椒和竹叶花椒中的平均加样回收率(n=6)分别为99.3%~105.1%和91.9%~99.8%。花椒和竹叶花椒中4个黄酮成分含量的相对平均偏差分别在0.05%~3.37%和0.02%~3.28%,QAMS法所得结果与外标法(ESM)接近。结论:该方法准确可靠,花椒中黄酮总量显著高于竹叶花椒,可为全面评价花椒和竹叶花椒的质量研究提供参考。Objective:To establish the quantitative analysis of multi-components by single marker(QAMS)meth⁃od to simultaneously determine the contents of rutin,hyperoside,isoquercetin and quercetin in Zanthoxyli Pericarpium and Pericarpium Zanthoxyli Armati,and compare the differences in flavonoids contents.Methods:Methanol extract of Zanthoxyli Pericarpium was analyzed on an Agilent Eclipse Plus C18(250 mm×4.6 mm,5μm)chromatographic column.The mobile phase was acetonitrile(A)-0.1%formic acid(B)in a gradient elution.The flow rate was 1.0 mL·min-1.The column temperature was 35℃,and the detection wavelength was 360 nm.The injection volume was 10μL.Using hyperoside as the internal standard substance,the relative correction factors for the other 3 components were calculate and the flavonoids contents in Zanthoxyli Pericarpium and Pericarpium Zanthoxyli Armati were determined.Results:The 4 components had good linear relationship within their respective ranges(r≥0.9999).The average recovery rates of Zanthoxyli Pericarpium were 99.3%-105.1%(n=6),while thoes of Pericarpium Zanthoxyli Armati were 91.9%-99.8%(n=6).The relative average deviations of content of Zanthoxyli Pericarpium were 0.05%-3.37%,and the relative average deviations of content of Pericarpium Zanthoxyli Armati were 0.02%-3.28%.The results obtained by QAMS method were close to those obtained by the external standard method(ESM).Conclusion:This method of QAMS method is accurate and reliable.The total amount of flavonoids in Pericarpium Zanthoxyli Armati is significantly higher than that in Zanthoxyli Pericarpium.Which provides reference for the comprehensive evaluation of the quality research of Zanthoxyli Pericarpium and Pericarpium Zanthoxyli Armati.

关 键 词：一测多评 花椒 竹叶花椒 黄酮成分 金丝桃苷 芦丁 异槲皮苷 槲皮苷 含量差异 

分 类 号：R917[医药卫生—药物分析学]