推拿对颈型颈椎病炎性痛的镇痛作用及对PI3K/AKT/mTOR通路RNA表达水平的影响  

Analgesic Effect of Massage on Inflammatory Pain in the Neck Type of Cervical Spondylosis and Its Effect on RNA Expression Level of PI3K/AKT/mTOR Pathway

作　　者：陈进城 林志刚 何坚 陈水金 江煜 林荣 杨一涵 陈乐春 张幻真 蒋晶晶 谷俊刚 程静 杨珊莉 CHEN Jincheng;LIN Zhigang;HE Jian;CHEN Shuijin;JIANG Yu;LIN Rong;YANG Yihan;CHEN Lechun;ZHANG Huanzhen;JIANG Jingjing;GU Jungang;CHENG Jing;YANG Shanli(Fujian Rehabilitation Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350003,China;RehabilitationTechnology Key Laboratory of Fujian Province,Fuzhou 350003,China;College of Rehabilitation Medicine Affiliated to Fujian Universityof Traditional Chinese Medicine,Fuzhou 350003,China;Zhangzhou Health Vocational College,Zhangzhou 363000,China)

机构地区：[1]福建中医药大学附属康复医院,福州350003 [2]福建省康复技术重点实验室,福州350003 [3]福建中医药大学康复医学院,福州350122 [4]漳州卫生职业学院,漳州363000

出　　处：《中国医药指南》2024年第25期6-9,共4页Guide of China Medicine

基　　金：国家重点研发计划(2022YFC2009700);福建省自然科学基金项目(2020J01760);福建省康复技术重点实验室开放课题(KF2019015);福建省高校产学合作项目(2021Y4017)。

摘　　要：目的研究推拿治疗颈型颈椎病炎性痛的镇痛机制。方法将12只兔采用随机数字表法分为空白组、模型组、推拿组,模型组和推拿组用无创颈型颈椎病动物模型,推拿组采用推拿治疗,模型组和空白组不予干预。治疗后用HE染色观察颈后肌组织形态;经酶联免疫吸附试验(ELISA)测定血清肿瘤坏死因子(TNF-α)和白细胞介素1β(IL-1β)。经RT-qPCR检测各组PI3K、AKT、mTOR通路的mRNA表达水平。结果推拿组肌纤维断裂处炎症细胞比模型组逐渐减少,肌原纤维排列整齐。模型组与空白组相比,血清TNF-α、IL-1β含量增高(P<0.01);推拿组较模型组的表达均下降(P<0.05);模型组较空白组PI3K、AKT、mTOR的mRNA表达上升(P<0.05);推拿组mRNA表达水平较模型组降低(P<0.05)。结论推拿能够降低颈型颈椎病炎性痛模型炎症因子TNF-α、IL-1β水平,可能通过降低PI3K/AKT/mTOR通路mRNA的表达发挥镇痛作用。Objective Study of the analgesic mechanism of massage therapy for the treatment of inflammatory pain in the neck type of cervical spondylosis.Methods Twelve rabbits were divided into blank group,model group and massage group by using random number table method,non-invasive the neck type of cervical spondylosis animal model was used in model and massage group,massage treatment was used in massage group,and no intervention was made in model and blank group.After treatment,HE staining was used to observe the tissue morphology of posterior cervical muscles;ELISA was used to determine serum TNF-αand IL-1β.RT-qPCR was used to detect the mRNA expression levels of PI3K,AKT,and mTOR pathway in each group.Results Inflammatory cells at muscle fibre breaks in the nudging group were gradually reduced compared with the model group,and myogenic fibres were neatly arranged.Compared with the model group and the blank group,serum TNF-αand IL-1βcontent increased(P<0.01);the expression of both decreased in the nudging group compared with that of the model group(P<0.05);the mRNA expression of PI3K,AKT and mTOR increased in the model group compared with that of the blank group(P<0.05);the level of mRNA expression in the nudging group decreased compared with that of the model group(P<0.05).Conclusions Massage was able to reduce the levels of inflammatory factors TNF-αand IL-1βin an inflammatory pain model of the neck type of cervical spondylosis,and massage may play an analgesic role by decreasing the expression of mRNA of the PI3K/AKT/mTOR pathway.

关 键 词：颈型颈椎病 推拿 炎性痛 PI3K/AKT/mTOR通路 

分 类 号：R681.5[医药卫生—骨科学]