氧化应激对多囊卵巢综合征患者辅助生殖结局的影响  

Effects of Oxidative Stress on Assisted Reproductive Outcomes in Patients with Polycystic Ovary

作　　者：薛东芳 贾三三 彭爱萍 XUE Dongfang;JIA Sansan;PENG Aiping(Department of Reproductive Medicine,Linfen People's Hospital,Linfen 041000,China)

机构地区：[1]山西省临汾市人民医院生殖医学科,临汾041000

出　　处：《中国医药指南》2024年第25期27-31,共5页Guide of China Medicine

基　　金：临汾市人民医院2021年院级课题立项项目(T20210512143)。

摘　　要：目的分析氧化应激对多囊卵巢综合征(PCOS)患者辅助生殖技术(ART)结局的影响。方法选取2021年9月至2023年4月在我中心行体外受精-胚胎移植(IVF-ET)/卵胞浆内单精子显微注射技术(ICSI)助孕的80例PCOS不孕患者设为研究组,另选同期的44例单纯输卵管因素不孕者设为对照组,采集取卵日卵泡液,检测氧化应激相关指标:褪黑素(MET)、丙二醛(MDA)、总抗氧化能力(TAC),并分析各指标与ART结局的关系。结果研究组ART助孕过程中每日平均用药量为(1.69±0.48)支、获卵数(17.41±2.51)个、卵母细胞成熟率为72.3%、正常受精率为73.4%、可利用胚胎率72.4%,对照组ART助孕过程中每日平均用药量为(1.99±0.61)支、获卵数为(12.49±2.63)个、卵母细胞成熟率为86.7%、正常受精率为80.1%、可利用胚胎率89.9%。研究组ART助孕过程中卵母细胞成熟率、正常受精率、可利用胚胎率低于对照组,获卵数高于对照组(P<0.05)。研究组MET、TAC水平低于对照组,MDA水平高于对照组(P<0.05)。Spearman相关性分析结果表明,MET、MDA、TAC与ART结局均无相关性(P>0.05)。结论PCOS不孕患者与单纯输卵管因素不孕患者间氧化应激水平存在差异,PCOS不孕患者卵泡液氧化应激水平与ART治疗结局存在相关性,可对卵子质量产生影响,进而影响到ART结局。Objective To analyze the effects of oxidative stress on assisted reproductive technology(ART)outcomes in patients with polycystic ovary syndrome(PCOS).Methods Selected from September 2021 to April 2023 in vitro fertilization and embryo transfer,in vitro fertilization and embryo transfer,Eighty patients with PCOS infertility assisted by IVF-ET/intracytoplasmic sperm injection(ICSI)were selected as the study group,and 44 patients with simple tubal infertility were selected as the control group.Follicular fluid was collected on the day of egg retrieval.Melatonin(MET),malondialdehyde(MDA)and total antioxidant capacity(TAC)were detected,and the relationship between each index and ART outcome was analyzed.Results In the study group,the average daily dose of ART was(1.69±0.48),the number of eggs obtained was(17.41±2.51),the oocyte maturity rate was 72.3%,the normal fertilization rate was 73.4%,and the available embryo rate was 72.4%.In the control group,the average daily dose of ART was(1.99±0.61),the number of eggs obtained was(12.49±2.63),the oocyte maturity rate was 86.7%,the normal fertilization rate was 80.1%,and the available embryo rate was 89.9%.During ART assisted pregnancy,the oocyte maturation rate,normal fertilization rate and available embryo rate in the study group were lower than those in the control group,the number of eggs obtained was higher than that in the control group(P<0.05).The levels of MET and TAC in the study group were lower than those in the control group and the levels of MDA were higher than those in the control group(P<0.05).Spearman's correlation analysis showed that MET,MDA,and TAC were not correlated with ART outcomes(P>0.05).Conclusions There is a difference in the level of oxidative stress between PCOS infertility patients and tubal infertility patients.The level of follicular liquid oxygen stress in PCOS infertility patients is correlated with the outcome of ART treatment,which can affect the quality of eggs and thus the outcome of ART.

关 键 词：氧化应激 多囊卵巢综合征 辅助生殖技术 

分 类 号：R711.75[医药卫生—妇产科学] R714.8[医药卫生—临床医学]