基于表面增强拉曼光谱法的5-羟基色氨酸快速检测方法的研究  

Research on rapid detection method of 5-hydroxytryptophan based on surface-enhanced Raman spectroscopy

作　　者：杨帆 秦丽 常化仿 章祥 李文华 吴虎 孙伟 YANG Fan;QIN Li;CHANG Huafang;ZHANG Xiang;LI Wenhua;WU Hu;SUN Wei(Hebei Food Safety Key Laboratory,Key Laboratory of Special Food Supervision Technology for State Market Regulation,Hebei Engineering Research Center for Special Food Safety and Health,Hebei Food Inspection and Research Institute,Shijiazhuang Hebei 050227,China;Hebei Intellectual Property Protection Center,Shijiazhuang Hebei 050022,China;Polaris Scientific Instrument(Hebei)Co.,Ltd.,Shijiazhuang Hebei 050000,China;Evidence Identification Center of Zhangjiagang Public Security Bureau,Zhangjiagang Jiangsu 215600,China)

机构地区：[1]河北省食品安全重点实验室,国家市场监管重点实验室(特殊食品监管技术),特殊食品安全与健康河北省工程研究中心,河北省食品检验研究院,河北石家庄050227 [2]河北省知识产权保护中心,河北石家庄050022 [3]普拉瑞思科学仪器(河北)有限公司,河北石家庄050000 [4]张家港市公安局物证签定室,江苏张家港215600

出　　处：《河北省科学院学报》2024年第4期49-53,共5页Journal of The Hebei Academy of Sciences

基　　金：国家市场监督管理总局技术保障专项(2023 YJ15);中央引导地方科技发展资金项目(科技成果转移转化项目)(236Z1708G)。

摘　　要：建立了一种基于表面增强拉曼光谱法快速测定食品中5-羟基色氨酸(5-hydroxytryptophan,5-HTP)的方法。本方法采用6次平行实验,样品使用乙腈提取,1 M硝酸反萃后与增强试剂混合上机检测,可以观测到良好的增强拉曼信号,线性范围在0~20 mg/kg,检出限为1 mg/kg。使用该方法检测市售的样品,并结合平行实验验证,检测结果和HPLC方法有较高的一致性。该方法具有操作简单、快速、高效的特点。In this paper,a method for the rapid determination of 5-hydroxytryptophan based on surface-enhanced Raman spectroscopy is established,which has the characteristics of simplicity,fastness and efficiency.This method employs six parallel experiments,and the samples are extracted with acetonitrile,back-extracted with 1 M nitric acid and mixed with the enhancement reagent for on-line detection.A good enhanced Raman signal is observed.The linear range is 0~20 mg/kg,and the detection limit is 1 mg/kg.The above method is used to detect commercially available samples,and combined with parallel experimental verification,the detection results are highly consistent with the HPLC method.

关 键 词：表面增强拉曼光谱法 快速检测 5-羟基色氨酸(5-HTP) 

分 类 号：O657.37[理学—分析化学] TS207[理学—化学]