异氟烷抑制星形胶质细胞葡萄糖摄取  

Isoflurane Inhibits Glucose Uptake in Astrocytes in Vitro

作　　者：郝景茹[1] 王琥祎 高灿[1] HAO Jingru;WANG Huyi;GAO Can(NMPA Key Laboratory for Research and Evaluation of Narcotic and Psychotropic Drugs,Jiangsu Province Key Laboratory of Anesthesiology,Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application,Xuzhou Medical University,Xuzhou Jiangsu 221004)

机构地区：[1]国家药品监督管理局麻醉药品和精神药品研究与评价重点实验室,江苏省麻醉学重点实验室,江苏省麻醉与镇痛应用重点实验室,徐州医科大学,江苏徐州221004

出　　处：《中国科技纵横》2024年第11期126-128,共3页China Science & Technology Overview

基　　金：国家自然科学基金资助项目(82371215)。

摘　　要：本文探讨了异氟烷对星形胶质细胞葡萄糖摄取的影响,应用培养的大鼠星形胶质细胞,通过免疫荧光观察异氟烷对星形胶质细胞结构的影响;利用6-NBDG检测异氟烷对星形胶质细胞葡萄糖吸收影响;通过RT-PCR和免疫印迹检测葡萄糖转运体的变化。与对照组相比,异氟烷诱导GFAP向细胞膜处聚集,抑制星形胶质细胞葡萄糖摄取及GLuT1的表达。异氟烷可以通过抑制星形胶质细胞葡萄糖转运体的表达,抑制葡萄糖摄取。To investigate the effects of isoflurane on glucose uptake in astrocytes.Cultured rat astrocytes were exposed with isoflurane.Using immunofluorescence to examine the effect of isoflurane on astrocyte structure.Using 6-NBDG to detect the glucose absorption;Using RT-PCR and WB to detect the glucose transporters.Isoflurane induced the aggregation of GFAP to the cell membrane.Isoflurane inhibited glucose uptake,GLuT1 expression in astrocytes.Isoflurane may inhibit glucose transporters expression in astrocytes,resulting in impaired glucose uptake.

关 键 词：异氟烷 葡萄糖摄取 星形胶质细胞 葡萄糖转运体 

分 类 号：H09[语言文字—语言学]