常温机械灌注技术在离断肢体保存中的作用研究进展  

Research progress on the role of normothermic machine perfusion in the preservation of severed limbs

作　　者：贾志博 管延军 宋翔宇 董阳辉 杨博尧 崔梦一 许文静 彭江 Jia Zhibo;Guan Yanjun;Song Xiangyu;Dong Yanghui;Yang Boyao;Cui Mengyi;Xu Wenjing;Peng Jiang(Research Institute of Orthopaedic Medicine Department,the Fourth Medical Center of the Chinese People's Liberation Army,All-Army Orthopaedic Battle Trauma Key Laboratory,Beijing Key Laboratory of Orthopaedic Regenerative Medicine,Beijing 100853,China;不详)

机构地区：[1]中国人民解放军总医院第四医学中心骨科医学部研究所全军骨科战创伤重点实验室骨科再生医学北京市重点实验室,北京100853 [2]河北北方学院研究生院 [3]解放军医学院

出　　处：《器官移植》2024年第5期824-829,共6页Organ Transplantation

基　　金：国家重点研发计划(2019YFA0110704);解放军总医院青年自主创新科学基金项目(22QNFC014)。

摘　　要：肢体离断伤在临床工作中较为常见,对离断肢体实施安全有效的保护是肢体再植成功的关键。常温机械灌注技术已经在器官移植领域取得很大突破,可长时间维持器官和组织的活性功能,延长其保存时间,在大动物模型以及临床应用中得到了很好的验证。同时这项技术有望为离断肢体的保存和功能恢复提供新的参考。因此,本文就静态冷保存在离断肢体保存中存在的问题、机械灌注的发展历程、离断肢体常温机械灌注的临床应用现状以及有待解决问题进行综述,并展望其发展方向和临床应用前景,以期推动该技术在临床广泛应用。Limb dismemberment injuries are common in clinical practice,and safe and effective protection of the dismembered limb is the key to successful limb replantation.Normothermic machine perfusion has made significant breakthrough in the field of organ transplantation,which may maintain the active function of organs and tissues for a long period of time and prolong the preservation time.These findings have been validated in large animal models and clinical trials.Meantime,this technology is expected to provide novel reference for the preservation and functional recovery of severed limbs.Therefore,this paper reviews the problems of static cold preservation in the preservation of disarticulated limbs,the development history of mechanical perfusion,the current status of clinical application of ambient mechanical perfusion of disarticulated limbs as well as the problems to be solved,and looks forward to the direction of its development and the prospect of its clinical application,with a view to promoting the wide application of this technology in the clinic.

关 键 词：离断肢体 肢体保存 静态冷保存 常温机械灌注 肢体再植 缺血-再灌注损伤 灌注液 含氧载体 

分 类 号：R617[医药卫生—外科学] R658[医药卫生—临床医学]