哮喘急性发作小儿HRV感染及嗜酸粒细胞与总IgE表达  

HRV infection and expression of eosinophils and total IgE in children with acute asthma attack

作　　者：乔在霞 隋英 詹莉芳 冯晓 徐镇阔 陈红营 QIAO Zai-xia;SUI Ying;ZHAN Li-fang;FENG Xiao;XU Zhen-kuo;CHEN Hong-ying(Dezhou Hospital,Qilu Hospital,Shandong University,Dezhou,Shandong 253000,China;不详)

机构地区：[1]山东大学齐鲁医院德州医院儿童呼吸与危重症科,山东德州253000 [2]山东大学齐鲁医院德州医院儿科,山东德州253000 [3]山东大学齐鲁医院德州医院检验科,山东德州253000 [4]德州恒丰医院全科医学科,山东德州253000

出　　处：《中华医院感染学杂志》2024年第16期2505-2509,共5页Chinese Journal of Nosocomiology

基　　金：山东省医药卫生科技发展计划基金资助项目(202206031088)。

摘　　要：目的探究哮喘急性发作小儿人鼻病毒(HRV)感染情况及嗜酸粒细胞(EOS)、总免疫球蛋白E(IgE)的表达情况。方法选取山东大学齐鲁医院德州医院2022年1月-2023年10月间186例哮喘急性发作患儿为研究对象,另选取同期120名健康体检患儿为对照组,比较两组基线资料和血清EOS、IgE表达情况,采用荧光定量聚合酶链式反应(PCR)法检测研究组患儿HRV感染情况,根据检测结果将其分为HRV感染组(n=90)和非感染组(n=96),分析研究组不同年龄阶段患儿HRV感染情况,比较两组血清EOS、IgE水平和临床肺部感染评分(CPIS),根据疾病严重程度将HRV感染哮喘患儿分为轻度组(n=52)、中重度组(n=38),比较两组患儿EOS、IgE水平和CPIS评分,采用Pearson相关系数分析血清EOS、总IgE水平和CPIS评分的相关性,采用受试者工作特征(ROC)曲线分析EOS、IgE水平对哮喘急性发作小儿HRV感染的诊断价值。结果研究组EOS、总IgE水平高于对照组(P<0.05);186例患儿中经检测HRV阳性90例,阴性96例,感染率为48.39%,其中感染率较高的年龄阶段为0~1岁及2~3岁,分别为48.21%、61.19%;HRV感染患儿EOS、总IgE水平和CPIS评分高于非感染患儿(P<0.05);轻度组EOS、总IgE水平和CPIS评分低于中重度组(P<0.05);Pearson相关系数分析显示,血清EOS、总IgE水平与CPIS评分均呈正相关(P<0.05);ROC曲线分析显示,EOS、总IgE水平联合预测哮喘急性发作患儿HRV感染的敏感度为91.11%,特异度为62.50%,曲线下面积(AUC)为0.908,联合诊断价值更高。结论哮喘急性发作患儿HRV感染率较高,血清EOS、总IgE水平也明显升高,且EOS、总IgE水平随病情加重而升高,二者对HRV感染具有较高的诊断价值。OBJECTIVE To explore the human rhinovirus(HRV)infection status and the expression of eosinophil(EOS)and total immunoglobulin E(IgE)in children with acute attack of asthma.METHODS A total of 186 children with acute attack of asthma in Dezhou Hospital of Qilu Hospital in Shandong University from Jan 2022 to Oct 2023 were enrolled as the research subjects,and 120 healthy children under physical examination during the same period were included in the control group.The baseline data and expression of serum EOS and IgE were compared between the two groups.The HRV infection status in children of the study group was detected by fluorescence quantitative polymerase chain reaction(PCR).According to the detection results,they were divided into the HRV infection group(n=90)and non-infection group(n=96),HRV infection in children of different ages of the study group was analyzed,and the serum EOS and IgE levels and clinical pulmonary infection score(CPIS)were compared between the two groups.According to disease severity,children with HRV infection and asthma were classified into the mild group(n=52)and moderate-to-severe group(n=38),and the levels of EOS and IgE and CPIS score were compared between the two groups.Pearson correlation coefficient was used to analyze the correlation between serum EOS,total IgE and CPIS score.Receiver operating characteristic(ROC)curve was adopted to analyze the diagnostic value of EOS and IgE levels on HRV infection in children with acute attack of asthma.RESULTS The levels of EOS and total IgE in the study group were significantly higher than those in the control group(P<0.05).Among the 186 children,90 cases were positive for HRV and 96 cases were negative,with an infection rate of 48.39%.Patients aged between 0-1 and 2-3 years had higher infection rates,accounting for 48.21%and 61.19%,respectively.The levels of EOS and total IgE and score of CPIS in the HRV infection group were higher than those in the non-infection group(P<0.05).The EOS and total IgE levels and CPIS score were lower in the mil

关 键 词：哮喘 急性发作 人鼻病毒 嗜酸粒细胞 总免疫球蛋白E 

分 类 号：R725.6[医药卫生—儿科]