Toxoplasma gondii adhesion and apoptosis of chicken erythrocytes  

作　　者：Chenghuan Li Xiaohan Zhang Xiaoyu Sang Yanhong He Saeed El-Ashram Yingying Ding Tiantian Jiang Na Yang 

机构地区：[1]Key Laboratory of Livestock Infectious Diseases in Northeast China,Ministry of Education,Key Laboratory of Zoonosis,College of Animal Science and Veterinary Medicine,Shenyang Agricultural University,Shenyang,China [2]Faculty of Science,Kafrelsheikh University,Kafr El-Sheikh,Egypt [3]Department of Pediatrics,School of Medicine,University of California,La Jolla,San Diego,CA,USA

出　　处：《Animal Diseases》2023年第3期178-187,共10页动物疾病（英文）

基　　金：This work was supported by grants from the National Key Research and Development Program of China(2022YFD1800200);the National Natural Science Foundation of China(Grant Numbers 32072891,31672546,31902297);Education Department of Liaoning Province Project(LSNQN202003,LUKZ0673);Shenyang Young and middle-aged Scientific and technological Innovation Talent Support Program(RC210291);Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases,Ministry of Agriculture and Rural Affairs,PR.China and Key Laboratory of Livestock Disease Prevention of Guangdong Province(YDWS202209).

摘　　要：Toxoplasma gondi is thought to infect all nucleated cells in warm-blooded animals,including poultry,mammals,and humans.However,it is unclear whether T.gondi can infect chicken erythrocytes due to the nucleated nature of these cells.Due to the special role of chicken erythrocytes in innate immunity,we investigated the cell-cell interaction between T.gondi and erythrocytes to elucidate the role of chicken erythrocytes in T.gondi infection.Cellular apoptosis was analyzed by transwell assay and flow cytometry.An immunofluorescence method was used to examine the reorganization of vimentin during T.gondi infection in both Vero cells and chicken erythrocytes.The reorganization of actin was evaluated to further examine the invasion capacity of tachyzoites on chicken erythrocytes during infection.We discovered that T.gondi can adhere to but not invade chicken erythrocytes and eventually cause apoptosis in chicken erythrocytes.When tachyzoites were cocultured with chicken erythrocytes in vitro,the transcrip-tional levels of T.gondi MIC3,ROP16,and ROP18 were significantly decreased.In addition,the rearrangement of host cell vimentin,a type Il cytoskeleton protein regulated by T.gondii infection,was not observed.Similarly,the parasite-induced ring-shaped actin structure was not formed in the host-parasite junction.T.gondi(RH strain)tachyzoites pref-erentially invaded Vero cells and replicated in chicken blood monocytes,but they were not found in chicken erythro-cytes.These findings showed that although T.gondi could attach to the surface of chicken erythrocytes,but couldn't invade successfully.Interestingly,we found that the T.gondii secretome,lysates,and intact tachyzoites could cause apoptosis of chicken erythrocytes,which suggested a complex mechanism involved in the apoptosis of chicken erythrocytes induced by T.gondi.This study elucidated that T.gondi could not infect nucleated chicken erythrocytes and enriched our understanding of the transmission mechanism of T.gondii among avian species.

关 键 词：Toxoplasma gondi Chicken erythrocytes INVASION APOPTOSIS 

分 类 号：S855.9[农业科学—临床兽医学]