α-1,6葡聚糖与低聚异麦芽糖对益生菌和人体肠道菌群的影响比较研究  

Comparative study on the effects ofα-1,6-glucan and oligomeric isomaltose on probiotics and human gut microbiota

作　　者：杨玉洁 吴迪楠 李宇轩 何莹 胡晓飞 孙雯颖 李彧媛[2] 李明[2] 鞠伟伟 YANG Yujie;WU Dinan;LI Yuxuan;HE Ying;HU Xiaofei;SUN Wenying;LI Yuyuan;LI Ming;JU Weiwei(The First Affiliated Hospital of Anhui Medical University,Hefei,Anhui 230022,China;Dalian Medical University,Dalian,Liaoning 116044,China;不详)

机构地区：[1]安徽医科大学第一附属医院,安徽合肥230022 [2]大连医科大学 [3]广州华汇生物实业有限公司 [4]大连市中心医院 [5]大连医科大学附属第二医院

出　　处：《中国微生态学杂志》2024年第7期783-787,共5页Chinese Journal of Microecology

摘　　要：目的比较α-1,6葡聚糖和低聚异麦芽糖对益生菌的体外增殖作用,评价2种低聚糖对人体肠道菌群的影响。方法脆弱拟杆菌在分别含有2种低聚糖的不同培养基中发酵,活菌计数绘制生长曲线;采集12位健康志愿者的新鲜粪便,将肠道菌群悬液按10%的接种量分别接种于Control组(含氮基础培养基)、α-1,6葡聚糖组(含氮基础培养基加1%α-1,6葡聚糖)及低聚异麦芽糖组(含氮基础培养基加1%低聚异麦芽糖),37℃恒温静态厌氧发酵24 h。采用高通量测序技术分析粪便发酵液菌群结构;应用高效液相色谱法检测粪便发酵液中短链脂肪酸(SCFAs)水平。结果α-1,6葡聚糖促进脆弱拟杆菌增殖的能力较低聚异麦芽糖强[(5.27±0.33)vs(4.80±0.66),×10^(8)CFU/mL];粪便发酵实验显示α-1,6葡聚糖可降低粪便中厚壁菌门/拟杆菌门比值(t=2.821,P=0.0478),显著提升SCFAs水平(丙酸:t=3.750,P=0.0092;丁酸:t=5.747,P=0.0018)。结论α-1,6葡聚糖可以改善肠道菌群结构并促进SCFAs产生。Objective To compare the effects ofα-1,6-glucan and oligomeric isomaltose on probiotics and human gut microbiota.Methods Bacteroides fragile ferments in different media containing two types of oligosaccharides,and the growth curve was plotted by counting the live bacteria;Fresh feces from 12 healthy volunteers were collected,and the suspension of gut microbiota were inoculated to the control group(nitrogen-containing basic culture medium),α-1,6-glucan group(nitrogen-containing basic culture medium with 1%α-1,6-glucan),and oligosaccharide isomaltose group(nitrogencontaining base medium with 1%oligosaccharide isomaltose)at a 10%inoculation rate.The static anaerobic fermentation was carried out for 24 hours under constant temperature conditions at 37℃.High-throughput sequencing technology was used to analyze the microbial community structure of fecal fermentation broth;Short chain fatty acids(SCFAs)levels in fecal fermentation broth were detected by High Performance Liquid Chromatography(HPLC).Resultsα-1,6-glucan had a stronger ability to promote the proliferation of Bacteroides fragile compared to oligomeric isomaltose[(5.27±0.33)vs(4.80±0.66),×10^(8)CFU/mL];Reduce the ratio of Firmicutes/Bacteroidetes in feces(t=2.821,P=0.0478)and significantly increase the content of SCFAs(Propionate:t=3.750,P=0.0092;Butyrate:t=5.747,P=0.0018).Conclusionα-1,6-glucan can improve the structure of gut microbiota and promote the production of SCFAs,which may alleviate intestinal inflammation and alleviate obesity.

关 键 词：α-1 6葡聚糖 低聚异麦芽糖 肠道菌群 短链脂肪酸 

分 类 号：R539[医药卫生—内科学]