利用转录组测序技术在大连地区发现类鼻疽伯克霍尔德菌感染病例  

Burkholderia pseudomallei infection case was found in Dalian by transcriptome sequencing

作　　者：栾明春[1] 王越[1] 郎兴莹[1] 薄志坚[1] LUAN Mingchun;WANG Yue;LANG Xingying;BO Zhijian(Dalian Center for Disease Control and Prevention,Dalian,Liaoning 116035,China)

机构地区：[1]大连市疾病预防控制中心,辽宁116035

出　　处：《中国微生态学杂志》2024年第7期813-816,共4页Chinese Journal of Microecology

基　　金：大连市科技创新基金项目(2022JJ13SN082)。

摘　　要：目的利用转录组测序技术直接检测疑似感染患者标本中的未知病原体,为感染的诊断、治疗和预警提供依据。方法对大连地区疑似感染的一名患者的标本进行RNA提取,直接用于RNA文库构建和测序,对测序获得数据进行生物信息学分析,与微生物数据库进行比对。为验证可疑病原体,对标本进行分离培养和特异性PCR扩增。结果经分析,该患者标本有2216条序列reads与类鼻疽伯克霍尔德菌同源,验证结果显示从标本中分离到鼻疽/类鼻疽伯克霍尔德菌,并从标本中特异扩增出类鼻疽伯克霍尔德菌的序列而未扩增出鼻疽伯克霍尔德菌的序列,提示患者体内存在类鼻疽伯克霍尔德菌感染。结论首次在大连地区检出类鼻疽病例。转录组测序技术可直接检测疑似感染患者体内未知病原体,可为传染病等突发公共卫生事件的预警、监测和处置提供有力手段。Objective To directly detect unknown pathogens in specimen of patient with suspected infection by using transcriptome sequencing,providing a basis for diagnosis,treatment and early warning of infection.Methods The RNA was extracted from the specimen of a patient with suspected infection in Dalian area.The RNA-Seq library based on the Ion Torrent second generation sequencing was established and sequenced.The sequencing data was analyzed using bioinformatics to identify the specie of microorganisms present in the specimen,so as to identify the pathogens and verify them with isolation and PCR.Results The sequencing data analysis found that 2,216 sequence reads were homologous to Burkholderia pseudomallei.The verification results showed that Burkholderia mallei/Burkholderia pseudomallei was isolated from the specimen,and Burkholderia pseudomallei sequences were specifically amplified from the specimen without amplification of Burkholderia mallei,suggesting the presence of Burkholderia pseudomallei infection in the patient.Conclusion The melioidosis was detected in Dalian area for the first time.Transcriptome sequencing can be used to directly detect unknown pathogens in patients with suspected infection,provides a powerful technical means for early warning,monitoring and handling of public health emergencies such as infectious diseases.

关 键 词：转录组测序 类鼻疽伯克霍尔德菌 未知病原体 

分 类 号：R372[医药卫生—病原生物学]