电催化制过氧化氢消毒液物种成分分析及消毒性能观察  

Analysis of active ingredients and observation of microbial disinfection performance of electrocatalytic hydrogen peroxide disinfectant

作　　者：赵思栎 杨金星 刘羽翀 左国民 齐丽红 ZHAO Si-yue;YANG Jin-xing;LIU Yu-chong;ZUO Guo-min;QI Li-hong(Institute of NBC Defense,PLA Army,Beijing 102205,China)

机构地区：[1]中国人民解放军陆军防化学院,北京102205

出　　处：《中国消毒学杂志》2024年第8期579-582,586,共5页Chinese Journal of Disinfection

摘　　要：目的探索电催化制过氧化氢过程物种成分并观察其对3种指示菌的消毒效果。方法采用气体扩散流动电解池制备过氧化氢消毒液,利用电子自旋共振(EPR)检测活性氧种类及变化规律,并与市售过氧化氢对比,观察对大肠埃希菌、金黄色葡萄球菌和枯草杆菌黑色变种芽孢3种微生物指示菌的消毒效果。结果电催化制过氧化氢消毒液中除过氧化氢外,还存在超氧阴离子、羟基自由基和单线态氧3种亚稳态活性氧,其比例约为5:3:1,且在电催化制结束后约1 h内3种新生态活性氧含量均可维持在较高水平;电催化制过氧化氢消毒液对大肠埃希菌、金黄色葡萄球菌和枯草杆菌黑色变种芽孢在消毒作用1、5和30 min时对3种微生物指示菌能满足杀灭对数大于5.0的消毒作业要求。结论电催化制过氧化氢消毒液在产生过氧化氢同时生成多种活性氧,可以有效提高杀灭微生物能力。Objective To analyze the active species in the process of electrocatalytic production of hydrogen peroxide and to observe their disinfection performance on three indicator bacteria.Methods Hydrogen peroxide was prepared using a gas diffusion flow electrolysis cell.The reactive oxygen species were detected using electron spin resonance(EPR),and the disinfection performance on three microbial indicator bacteria,Escherichia coli,Staphylococcus aureus,and Bacillus subtilis black variety spores were observed as comparing to the commercially available hydrogen peroxide with same concentration.Results In addition to hydrogen peroxide,three metastable reactive oxygen species,namely superoxide anion,hydroxyl radical,and singlet oxygen existed in the electrocatalytic hydrogen peroxide disinfection solution,with a ratio of about 5:3:1.The content of the three new ecological reactive oxygen species could be maintained at a high level within about 1 h after the end of the electrocatalytic process.The electrocatalytic hydrogen peroxide disinfectant could meet the disinfection requirements of killing logarithms greater than 5.0 for three microbial indicator bacteria,including Escherichia coli,Staphylococcus aureus,and Bacillus subtilis black variant spores,after 1 min,5 min,and 30 min of disinfection.Conclusion The electrocatalytic production of hydrogen peroxide disinfectant can effectively improve the ability to kill microorganisms by generating hydrogen peroxide and generating various reactive oxygen species.

关 键 词：电催化 过氧化氢 活性氧 细菌 消毒 

分 类 号：R187[医药卫生—流行病学]