CONSTANS alters the circadian clock in Arabidopsis thaliana  

作　　者：Pedro de los Reyes Gloria Serrano-Bueno Francisco J.Romero-Campero He Gao Jose M.Romero Federico Valverde 

机构地区：[1]Plant Development Group-Institute for Plant Biochemistry and Photosynthesis,Consejo Superior de Investigaciones Cientificas,Universidad de Sevilla,Seville,Spain [2]Department of Computer Science and Artificial Intelligence,Universidad de Sevilla,Seville,Spain [3]Department of Plant Developmental Biology,Max Planck Institute for Plant Breeding Research,Cologne,Germany [4]Department of Plant Biochemistry and Molecular Biology,Universidad de Sevilla,Seville,Spain

出　　处：《Molecular Plant》2024年第8期1204-1220,共17页分子植物（英文版）

基　　金：a European Union contract LONGFLOW,MSCAIF-2018-838317 and CSIC LONGFLOW,CONV_EXT_014.The financial support of the Spanish Ministry for Science and Innovations(MICINN/FEDER)PID2020-117018RB-I00 to F.V.is also acknowledged.We thank Prof.George Coupland(MPiPZ,Cologne,Germany)for discussion and critical reading of the manuscript.

摘　　要：Plants are sessile organisms that have acquired highly plastic developmental strategies to adapt to the environment.Among these processes,the floral transition is essential to ensure reproductive success and is finelyregulated by several internal andexternal genetic networks.The photoperiodic pathway,which controls plant response to day length,is one of the most important pathways controlling flowering.In Arabidopsis photoperiodic flowering,CONSTANS(CO)is the central gene activating the expression of the florigen FLOWERING LOCUS T(FT)in the leaves at the end of a long day.The circadian clock strongly regulates Co expression.However,to date,no evidence has been reported regarding a feedbackloop from the photoperiod pathway back to the circadian clock.Using transcriptional networks,we have identified relevant network motifs regulating the interplay between the circadian clock and the photoperiod pathway.Gene expression,chromatin immunoprecipitation experiments,and phenotypic analysis allowed us to elucidate the role of Co over the circadian clock.Plants with altered cO expression showed a different internal clock period,measured by daily leaf rhythmic movements.We showed that co upregulates the expression of key genes related to the circadian clock,such as CCA1,LHY,PRR5,and Gl,at the end of a long day by bindingto specific sites on their promoters.Moreover,a high numberof PRR5-repressed target genes are upregulated by CO,and this could explain the phase transition promoted by CO.The CO-PRR5 complex interacts with the bZiP transcriptionfactor HY5andhelps to localize the complex in the promoters of clock genes.Taken together,our results indicate that there may be a feedback loop in which co communicates back to the circadian clock,providing seasonal information to the circadian system.

关 键 词：CONSTANS circadian clock PHOTOPERIOD 

分 类 号：Q94[生物学—植物学]